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Callus induction and adventitious organogenesis of kenaf (Hibiscus cannabinus L.)

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Abstract

Callus production along with caulogenesis and rhizogenesis were obtained from internodal stem explants of kenaf (Hibiscus cannabinus L.) after 4 weeks in culture. Murashige and Skoog medium was used for two 4×4 matrix experiments designed to determine suitable growth regulator combinations (NAA/BAP or 2,4-D/kinetin) and concentrations (0.1, 0.3, 1.0, 3.0 mg/L). The most abundant callus production was observed at 0.3/3.0 and 1.0/3.0 mg/L 2,4-D/kinetin and at 1.0/1.0 and 3.0/1.0 mg/L NAA/BAP. Rhizogenesis was most extensive with NAA/BAP at concentrations of 0.1/3.0 and 0.3/ 3.0 mg/L. Adventitious shoots developed on both auxin/cytokinin matrixes when each concentration was at 0.3 mg/L or less. These protocols will facilitate the development of in vitro approaches to kenaf improvement and the study of certain host-pathogen interactions.

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Abbreviations

MS:

Murashige and Skoog (1962) medium

2,4-D:

2,4-dichlorophenoxyacetic acid

BAP:

6-benzylaminopurine

NAA:

1-naphthyleneacetic acid

SDS:

sodium dodecyl sulfate

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Authors and Affiliations

  1. Department of Agriculture, Northeast Louisiana University, 71209, Monroe, LA, USA

    K. S. McLean

  2. Department of Plant Pathology and Weed Science, Mississippi State University, 39762, Mississippi State, MS, USA

    G. W. Lawrence

  3. Department of Horticulture, Mississippi State University, 39762, Mississippi State, MS, USA

    N. A. Reichert

Authors
  1. K. S. McLean
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  2. G. W. Lawrence
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  3. N. A. Reichert
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Additional information

Communicated by G. C. Phillips

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McLean, K.S., Lawrence, G.W. & Reichert, N.A. Callus induction and adventitious organogenesis of kenaf (Hibiscus cannabinus L.). Plant Cell Reports 11, 532–534 (1992). https://doi.org/10.1007/BF00236272

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  • DOI: https://doi.org/10.1007/BF00236272

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