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Presynaptic and postsynaptic organelles of synapses formed in cultures of previously dissociated mouse spinal cord

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Summary

This study describes some of the ultrastructural features of presynaptic and postsynaptic organelles at synapses developed in cultures of previously dissociated mouse spinal cord cells. Particular attention was paid to the agranular reticulum which is well developed at many presynaptic and postsynaptic sites, either in the form of simple tubules or cisternae, or more complex networks and often closely associated with mitochondria. In addition, the disposition of microtubules at and close to synaptic specializations is described. These and other features of synaptic zones, such as granular vesicles in presynaptic sites, are discussed in relation to cultures developed on feeder layers and synapses in vivo, and in relations to possible degenerative and regenerative events in the cell cultures.

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References

  • Bird, M.M.: Microtubule — synaptic vesicles associations in cultured rat spinal neurons. Cell Tiss. Res. 168, 101–115, (1976)

    Google Scholar 

  • Bird, M.M., James, D.W.: The development of synapses in vitro between previously dissociated chick spinal cord neurons. Z. Zellforsch. 140, 202–216 (1973)

    Google Scholar 

  • Bird, M.M., James, D.W.: An ultrastructural and electrophysiological study of the development of neuro-muscular juntions between previously dissociated foetal rat cells in vitro. Cell Tiss. Res. 155, 269–282 (1974)

    Google Scholar 

  • Bird, M.M., James, D.W.: The culture of previously dissociated embryonic chick spinal cord cells on feeder layers of liver and kidney, and the development of paraformaldehyde induced fluorescence upon the former. J. Neurocytol. 4, 633–646 (1975)

    Google Scholar 

  • Bird, M.M., James, D.W.: The development and ultrastructure of previously dissociated foetal human cerebral cortical cells in vitro. Cell Tiss. Res. 183, 403–417 (1977)

    Google Scholar 

  • Gonatas, N.K., Anderson, W., Evangelista, B.A.: The contribution of altered synapses in the senile plaque: and electron microscope study in Alzheimer's dementia. J. Neuropath. and Exp. Neurol. 26, 25–39 (1967)

    Google Scholar 

  • Gray, E.G.: Electron microscopy of presynaptic organelles of the spinal cord. J. Anat. 97, 101–106 (1963)

    Google Scholar 

  • Gray, E.G.: Presynaptic microtubules and their associations with synaptic vesicles. Proc. Roy. Soc. B. 190, 269–372 (1975)

    Google Scholar 

  • Gray, E.G.: Microtubules in synapses of the retinae. J. Neurocytol. 5, 361–370 (1976)

    Google Scholar 

  • Gray, E.G., Guillery, R.: Synaptic morphology in the normal and degenerating nervous system. Int. Cytol. 19, 111–182 (1966)

    Google Scholar 

  • Masurovsky, E.B., Benitez, H.H., Murray, M.R.: Synaptic development in long-term organized cultures or murine hypothalamus. J. Comp. Neurol. 143, 263–278 (1971)

    Google Scholar 

  • Malus, A.I., Walters, B.B., Mughal, S.: Immunohistochemical demonstration of tubulin associated with microtubules and synaptic junctions in mammalian brain. J. Neurocytol. 4, 733–744 (1975)

    Google Scholar 

  • Meller, K., Breipohl, W., Wagner, H., Knutz, A.: On the differentiation of isolated nerve and glial cells from trypsinized spinal cord of chick embryos in vitro. Z. Zellforsch. 101, 135–151 (1969)

    Google Scholar 

  • Nelson, P.G.: Central nervous system synapses in cell culture. Cold Spring Harbor Symp. Quant. Biol. 40, 277–287 (1976)

    Google Scholar 

  • Palay, S.L., Chan-Palay, V.: Cerebellar cortex cytology and organization. Berlin-Heidelberg-New York: Springer 1974

    Google Scholar 

  • Pappas, G.D., Waxman, S.G.: Synaptic fine structure — morphological correlates of chemical and electrotonic transmission. In Structure and Function of Synapses. (G.D. Pappas and D.P. Purpura, eds.), pp. 1–43. New York: Raven Press 1971

    Google Scholar 

  • Peters, A., Palay, S.L., Webster, H.deF.: The fine structure of the nervous system: the neurons and supporting cells. W.B. Saunders and Co. 1976

  • Sotelo, C., Palay, S.L.: Altered axons and axon terminals in the lateral vestibular nucleus of the rat. Lab. Invest. 25, 653–671 (1971)

    Google Scholar 

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  1. Department of Anatomy, The London Hospital Medical College, Turner Street, El 2AD, London, UK

    Margaret M. Bird

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  1. Margaret M. Bird
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Bird, M.M. Presynaptic and postsynaptic organelles of synapses formed in cultures of previously dissociated mouse spinal cord. Cell Tissue Res. 194, 503–511 (1978). https://doi.org/10.1007/BF00236169

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  • DOI: https://doi.org/10.1007/BF00236169

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