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Transient gene expression in strawberry (Fragaria x ananassa Duch.) protoplasts and the recovery of transgenic plants

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Summary

A transient ß-glucuronidase (GUS)-assay was performed to evaluate electroporation parameters and optimize DNA delivery conditions into strawberry protoplasts. Optimal GUS-activity was obtained when protoplasts were subjected to 400 V/cm for 20 ms. GUS-activity could be further increased by the addition of carrier DNA to the electroporation mixture. Callus selected on 10 μg/ml hygromycin produced shoots which exhibited GUS-activity. The transformed nature of the shoots obtained after selection was confirmed by DNA-analysis.

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Abbreviations

CaMV:

cauliflower mosaic virus

dCTP:

deoxycytidine-triphosphate

EtBr:

ethidium bromide

GUS:

ß-glucuronidase

MES:

2(N-morpholino) -ethanesulfonic acid

X-gluc:

5-bromo-4-chloro-3-indolyl glucuronide

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Authors and Affiliations

  1. Department of Physiological Botany, University of Uppsala, Box 540, S-751 21, Uppsala, Sweden

    Marie Nyman & Anita Wallin

Authors
  1. Marie Nyman
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  2. Anita Wallin
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Communicated by H. Lörz

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Nyman, M., Wallin, A. Transient gene expression in strawberry (Fragaria x ananassa Duch.) protoplasts and the recovery of transgenic plants. Plant Cell Reports 11, 105–108 (1992). https://doi.org/10.1007/BF00235264

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  • DOI: https://doi.org/10.1007/BF00235264

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