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Involvement of mixed function oxidase systems in polychlorinated biphenyl metabolism by plant cells

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Summary

Nineteen different polychlorinated biphenyl (PCB) congeners ranging in chlorine content from 2 to 6 chlorine atoms were provided to nonphotosynthetic suspension cultures of rose (Rosa sp. cv. Paul's Scarlet). After a 96 h incubation period, 11 individual congeners had been metabolized by > 10%. Provision of mixed function oxidase inhibitors (10 mM metyrapone or 0.5 mM 7,8-benzoflavone) either stopped or severely reduced the metabolism of individual congeners; whereas (inhibitors of peroxidase) (1 mM benzoate or 1 mM n-propylgallate) had minimal influence on PCB metabolism. The metabolism of PCBs by rose cultures appears to be catalyzed by a cytochrome P-450-and/or P-448-dependent enzyme system.

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Authors and Affiliations

  1. Department of Botany and Microbiology, University of Oklahoma, 73019, Norman, OK, USA

    Incheol Lee & John S. Fletcher

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  1. Incheol Lee
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  2. John S. Fletcher
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Communicated by J. M. Widholm

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Lee, I., Fletcher, J.S. Involvement of mixed function oxidase systems in polychlorinated biphenyl metabolism by plant cells. Plant Cell Reports 11, 97–100 (1992). https://doi.org/10.1007/BF00235262

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  • DOI: https://doi.org/10.1007/BF00235262

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