Summary
Embryogenic cell suspension cultures were established from Triticum aestivum X Leymus angustus F1 hybrids, using compact nodular calli derived from inflorescence segments. Calli originating from leaf segments did not give rise to stable cell suspensions. Growth measurements of the cell suspensions revealed that they continued rapid growth up to 10 days after subculturing. Flow cytometric studies of the cell cycle over a 7 day culture period showed that the majority of cells were in G1 phase while the rest were either in S or G2. During the 7 days of culture, no significant differences in DNA distribution patterns were observed. The cells from suspension cultures produced somatic embryos when they were transferred to different solid media. The embryos germinated and gave rise to plantlets which were successfully rooted and transferred to soil.
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References
Akagi H, Sakamoto M, Negishi T, Fugimura T (1989) Mol. Gen. Genet. 215: 501–506
Burnett PA (1984) In: Barley yellow dwarf virus, A proceedings of the workshop. CIMMYT, Mexico, pp 6–13
Comeau A, Plourde A (1987) Can. J. Plant Path. 9: 188–192
Green CE (1982) In: Fujiwara A (ed) Plant tissue culture. Maruzen, Tokyo, pp 107–108
Green CE, Armstrong CL, Anderson PC (1983) In: Dawney K, Weellmy RW, Ahmad F, Schultz J (eds) Advances in gene technology-molecular genetics of plants and animals. Academic Press, New-York, pp 147–157
Ho WJ, Vasil IK (1983) Ann. Bot. 51: 719–726
Karlsson SB, Vasil IK (1986) J. Plant Physiol. 123: 211–227
Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497
Ozias-Akins P, Ferl RJ, Vasil IK (1986) Mol. Gen. Genet. 203: 365–370
Paliwal YC, Comeau A (1987) Publication of Minestry of Agriculture and Food, Canada, Order no: 87-074
Plourde A (1988) Ph.D. Thesis, Laval University, Quebec, Canada
Redway AF, Vasil V, Vasil IK (1990) Plant Cell Rep. 8: 714–717
Tabaeizadeh Z, Ferl RJ, Vasil IK (1986) Proc. Natl. Acad. Sci. USA 86: 5616–5619
Tabaeizadeh Z, Plourde A, Comeau A (1990) Plant Cell Rep. 9: 204–206
Vasil IK (1988) Bio/Technol. 6: 397–402
Vasil V, Vasil IK (1981) Ann. Bot. 47: 669–678
Vasil V, Vasil IK (1986) J. Plant Physiol. 124: 399–408
Vasil V, Ferl RJ, Vasil IK (1988) J. Plant Physiol. 132: 160–163
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Tabaeizadeh, Z., Campeau, N. Embryogenic cell suspensions of Triticum aestivum x Leymus angustus F1 hybrids: characterization and plant regeneration. Plant Cell Reports 11, 81–85 (1992). https://doi.org/10.1007/BF00235258
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DOI: https://doi.org/10.1007/BF00235258