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The effect of growth retardants on anthocyanin production in carrot cell suspension cultures

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Abstract

The effect of growth retardants on anthocyanin production was studied in wild carrot (Daucus carota) cell suspension cultures. Paclobutrazol [(2RS,3RS) — 1 — (4-chlorophenyl) — 4,4 —dimethyl-2-(1,2,4-triazol-1-yl) pentan-3-ol], uniconazole [(E)-1-(4-chlorophenyl-4,4 —) dimethyl-2-(1,2,4-triazol-1-yl)-1-penten-3-ol], tetcyclacis [5-(4-chloro-phenyl) -3,4,5,9,10-pentaaza-tetracyclo-5, 4, 102,6, O8,11 — dodeca-3, 9-diene], ancymidol [α-cyclopropyl — 4 — methoxy-α(pyrimidine-5-yl)benzyl alcohol] and CCC (2-chloro-ethyltrimethylammonium chloride) increased anthocyanin accumulation. AMO-1618 [(2-isopropyl-5-methyl-4-trimethyl-ammonium-chloride)-phenyl-1-piperidinium carboxylate] did not increase anthocyanin accumulation in the first passage but did increase it during the second passage on medium for improved anthocyanin accumulation. Prohexadione (3,5-dioxo-4-propionylcyclohexane carboxylic acid) decreased anthocyanin accumulation by 10%–12.5%.

The inhibitory effect of gibberellin on anthocyanin accumulation was reversed by paclobutrazol. Paclobutrazol together with 10−6M GA3 increased anthocyanin level from 33% of control in GA3 treated cell suspension to 76%. These results are consistent growth retardants increasing anthocyanin accumulation in carrot cell suspension cultures by inhibiting gibberellin biosynthesis.

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Authors and Affiliations

  1. Department of Botany, The University of Tennessee, 37996, Knoxville, TN, USA

    Avihai Ilan & Donald K. Dougall

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  1. Avihai Ilan
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  2. Donald K. Dougall
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Communicated by A. R. Gould

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Ilan, A., Dougall, D.K. The effect of growth retardants on anthocyanin production in carrot cell suspension cultures. Plant Cell Reports 11, 304–309 (1992). https://doi.org/10.1007/BF00235087

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  • DOI: https://doi.org/10.1007/BF00235087

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