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Establishment of hairy root cultures of Linum flavum producing the lignan 5-methoxypodophyllotoxin

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Abstract

Hairy root cultures were induced from leaf explants of Linum flavum by infection with Agrobacterium rhizogenes. The transformed nature of tissue was confirmed by the production of opines. The cultures produced 1.5 to 3.5% of the lignan 5-methoxypodophyllotoxin (5-MPT) on a dry weight basis, which was 2 to 5 times higher than the 5-MPT content in untransformed root cultures and 5 to 12 times higher than in L. flavum cell suspensions. The 5-MPT production as a function of time was up to four times higher than that in cell suspensions.

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Abbreviations

NAA:

1-naphthaleneacetic acid

BAP:

6-benzylaminopurine

5-MPT:

5-methoxypodophyllotoxin

DW:

dry weight

GC-MS:

gas-chromatography coupled electron impact mass spectrometry

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Authors and Affiliations

  1. Department of Physiology and Biochemistry of Plants, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands

    André Oostdam

  2. Department of Physiology and Molecular Genetics, Vrije Universiteit, De Boelelaan 1087, 1081, HV Amsterdam, The Netherlands

    Jos N. M. Mol

  3. Department of Plant Physiology, Agricultural University, Arboretumlaan 4, 6703, BD Wageningen, The Netherlands

    Linus H. W. van der Plas

Authors
  1. André Oostdam
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  2. Jos N. M. Mol
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  3. Linus H. W. van der Plas
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Communicated by K. Hahlbrock

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Oostdam, A., Mol, J.N.M. & van der Plas, L.H.W. Establishment of hairy root cultures of Linum flavum producing the lignan 5-methoxypodophyllotoxin. Plant Cell Reports 12, 474–477 (1993). https://doi.org/10.1007/BF00234715

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  • DOI: https://doi.org/10.1007/BF00234715

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