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Callus induction and plant regeneration from explants of commercial cultivars of leek (Allium ampeloprasum var. porrum L.)

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Summary

Plant regeneration capacity was studied for 8 cultivars and 4 accessions of leek (A. ampeloprasum var. porrum L.). Compact callus was induced on embryo and leaf explants on three different media. The highest frequency of compact callus formation (up to 90%) was obtained when mature, zygotic embryos were cultured on MS medium, containing 30 g/l sucrose and 1 mg/l 2,4-D. Regeneration occurred through somatic embryogenesis on MS medium, supplemented with 1 mg/l kinetin. Plants could be regenerated from all cultivars and accessions tested. These cultivars and accessions could be classified into three groups with respect to shoot formation frequency. The results suggest a distinct influence of the genotype on the morphogenic response of leek embryo explants in vitro.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

MS:

Murashige and Skoog (1962) medium

N6 :

medium from Chu et al. (1975)

B5 :

medium from Gamborg et al. (1968)

BDS:

Dunstan and Short medium (1977)

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Author notes

  1. P. van der Valk

    Present address: VanderHave Research, P.O. Box 1, 4410, AA Rilland, The Netherlands

Authors and Affiliations

  1. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700, AA Wageningen, The Netherlands

    J. Buiteveld, P. van der Valk, J. Jansen, J. Creemers-Molenaar & C. M. Colijn-Hooymans

Authors
  1. J. Buiteveld
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  2. P. van der Valk
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  3. J. Jansen
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  4. J. Creemers-Molenaar
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  5. C. M. Colijn-Hooymans
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Communicated by H. Lörz

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Buiteveld, J., van der Valk, P., Jansen, J. et al. Callus induction and plant regeneration from explants of commercial cultivars of leek (Allium ampeloprasum var. porrum L.). Plant Cell Reports 12, 431–434 (1993). https://doi.org/10.1007/BF00234707

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  • DOI: https://doi.org/10.1007/BF00234707

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