Plant regeneration from isolated microspores of linseed (Linum usitatissimum L.)

Abstract

Embryogenesis and plant regeneration were induced in isolated microspore culture of linseed (oilflax, Linum usitatissimum). Microspores underwent cell divison which led to either microcallus or embryoid formation, when they were cultured in a modified liquid Nitsch-Lichter-Nitsch medium (Lichter 1985) at two different incubation temperatures (30 and 35 °C); some embryoids and microcalli further developed to larger calli. After transfer of the microspore derived calli to a solid medium containing zeatin (Img 1⁻¹) shoot induction was achieved from 36 to 66% of the calli. The highest frequency of regenerated plants was obtained in microspore cultures of the hybrid ‘Atalante’ x ‘Szegedi 62’ (F₁) at 30 °C, whereas for the second genotype ‘Pedigree 2’ x ‘Kiszombori 41’ (F₂) the higher incubation temperature seemed to be more efficient. Shoots could be successfully rooted on an indole acetic acid containing medium and then transplanted to vermiculite and finally to soil. Most of the plants survived the transfer into soil in the greenhouse, where they could be successfully grown to maturity.