Abstract
A high yielding cell line of Scutellaria baicalensis G. has successfully been developed to produce flavonoids. Major components of the flavonoids were identified as baicalin and wogonin-7-O-glucuronic acid by a series of instrumental analyses using UV, IR, MASS, and NMR. After 12 days in suspension culture, the cell growth reached 14 g DW/l, and baicalin and wogonin-7-O-glucuronic acid were obtained in concentrations of 2.9 g/l and 1.07 g/l, respectively. The culture temperature was found to be an important parameter for improving production yield of the flavonoids. The yield of baicalin was observed to increase to 4.2 g/l by shifting the temperature from 30 °C to 25 °C after 72 h of suspension culture.
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Abbreviations
- DW:
-
cell dry weight
- FW:
-
cell fresh weight
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- PSH medium:
-
phytohormone added Schenk and Hildebrandt medium
- FPM:
-
a modified Schenk and Hildebrandt medium for flavonoid production
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Communicated by F. Constabel
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Seo, W.T., Park, Y.H. & Choe, T.B. Identification and production of flavonoids in a cell suspension culture of Scutellaria baicalensis G.. Plant Cell Reports 12, 414–417 (1993). https://doi.org/10.1007/BF00234703
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DOI: https://doi.org/10.1007/BF00234703