Abstract
The Biolistics® particle delivery system for the transformation of soybean (Glycine max L. Merr.) was evaluated in two different regeneration systems. The first system was multiple shoot proliferation from shoot tips obtained from immature zygotic embryos of the cultivar Williams 82, and the second was somatic embryogenesis from a long term proliferative suspension culture of the cultivar Fayette. Bombardment of shoot tips with tungsten particles, coated with precipitated DNA containing the gene for β-glucuronidase (GUS), produced GUS-positive sectors in 30% of the regenerated shoots. However, none of the regenerants which developed into plants continued to produce GUS positive tissue. Bombardment of embryogenic suspension cultures produced GUS positive globular somatic embryos which proliferated into GUS positive somatic embryos and plants. An average of 4 independent transgenic lines were generated per bombarded flask of an embryogenic suspension. Particle bombardment delivered particles into the first two cell layers of either shoot tips or somatic embryos. Histological analysis indicated that shoot organogenesis appeared to involve more than the first two superficial cell layers of a shoot tip, while somatic embryo proliferation occurred from the first cell layer of existing somatic embryos. The different transformation results obtained with these two systems appeared to be directly related to differences in the cell types which were responsible for regeneration and their accessibility to particle penetration.
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Chee, P., Fober, K.A., and Slightom, J.L., (1989) Plant. Physiol. 91: 1212–1218
Christou, P. (1990a) Ann. Bot. 66 (4): 379–386
Christou, P. (1990b) Physiol. Plant. 79 (1): 210–212
Christou, P., McCabe, D.E., Martinell, B.J., and Swain, W.F., (1990) Trends Biotechnol. 8: 145–151
Christou, P., and McCabe, D.E., (1992) The Plant Journal. 2(3): 283–290
Church, G.M. and Gilbert, W. (1984) Proc. Natl. Acad. Sci. U.S.A. 81: 1991–1995
Dellaporta, S.L., Wood, J., and Hicks, J.B., (1983) Plant Mol. Bio. Reporter. 1: 19–21
Dos Santos, A.V.P., Cutter, E.G., and Davey, M.R. (1983) Protoplasma 117: 107–115.
Finer, J.J. and Nagasawa, A. (1988) Plant Cell, Tissue Organ Cult. 15: 125–136
Finer, J.J. and McMullen, M. (1991) In Vitro Cell. Dev Biol. 27P: 175–182.
Gamborg, O.L., Miller, R.A., Ojima, K. (1968) Exp. Cell Res. 50: 151–158
Hinchee, M.A., Connor-Ward, D.V., Newell, C.A., McDonnell, R. E., Sato, S., Gasser, C.S., Fischhoff, D.A., Re, D.B., Fraley, R.T., and, Horsch, R.B. (1988) Bio/Technology 6: 915–922
Jefferson, R.A., Burgess, S.M. and Hirsh, D. (1986) Proc. Natl. Acad. Sci. 83: 8447–8451
Johansen, D.A. (1940) McGraw-Hill, New York
Klein, T.M., Gradziel, T., Fromm, M.E., and Sanford, J.C. (1988) Bio/Technology 6: 559–563
Maataoui, M.E., Espagnac, H., and Michaux-Ferriere, N. (1990) Bot. 66: 183–190
Maheswaran, G. and Williams, E.G. (1985) Ann. Bot. 56: 619–630
McCabe, D.E., Swain, W.F., Martinell, B.J., and Christou, P., (1988) Bio/Technology 6: 923–926
Parrott, W.A., Hoffman, L.M., Hildegrand, D.F., Williams, E.G., and Collins, G.B, (1989) Plant Cell Rep. 7: 615–617
Sambrook, J., Fritsch, E.F.,and Maniatis T., 2nd edition. 1989
Southern, E.M. (1975) J. Mol. Biol. 98: 503–517
Sussex, I. (1989) Cell 56: 225–229
Taylor, M. and Vasil, I. (1991) Plant Cell Rep. 10: 120–125
Wang, Y., Klein, T.M., Fromm, M., Cao, J., Sanford, J.C., and Wu, R., (1988) Plant Mol. Biol. 11: 433–439
Zhou, J.H. and Atherly, A.G. (1990) Plant Cell Rep. 8 (9): 542–545
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Communicated by I. K. Vasil
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Sato, S., Newell, C., Kolacz, K. et al. Stable transformation via particle bombardment in two different soybean regeneration systems. Plant Cell Reports 12, 408–413 (1993). https://doi.org/10.1007/BF00234702
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DOI: https://doi.org/10.1007/BF00234702