Abstract
Vitrified shoots regenerated from carnation petals (Dianthus caryophyllus L. cv. Scania) were recovered by culturing them in a medium containing 3.0 g/l Bacto-Peptone. Wax structures not found on vitrified shoots developed on the abaxial surface of leaves of recovered shoots and on those of normal leaves. Recovered shoots were rooted and successfully acclimatized while vitrified shoots could not survive the acclimatization process. The Bacto-Peptone solution was fractionated and the efficiency of each fraction for the recovery of vitrification was examined. Only basic, non high molecular fractions whose molecular weight was less than 10,000 were effective.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Gimelli F, Ginatta G, Venturo R, Positan S, Buiatti M (1984) Riv Ortiflorofruttic 68:107–121
Hakkaart FA, Versluijs JMA (1983) Neth J Plant Path 89:47–53
Kakehi M (1979) Bulletin of The Hiroshima Agricultural College 6:159–166
Kever C, Coumans M, Coumans-Gilles MF, Gasper T (1984) Physiol Plant 61:69–74
Leshem B (1983a) Ann Bot 42:413–415
Leshem B (1983b) Ann Bot 52:873–876
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Orlikowska T (1987) Acta Hort 212:237–244
Rugini E (1986) Almonds. In: Handbook of Plant Cell Culture, Vol 4 (DA Evans et al.) eds Macmillan Pub. Co, London, pp 576–611
Sutter E, Langhans RW (1979) J Am Soc Hort Sci 104:493–496
Thomas WZ, Cobb BG (1989) Plant Cell Reports 8:358–360
Author information
Authors and Affiliations
Additional information
Communicated by A. Komamine
Rights and permissions
About this article
Cite this article
Sato, S., Hagimori, M. & Iwai, S. Recovering vitrified carnation (Dianthus caryophyllus L.) shoots using Bacto-Peptone and its subfractions. Plant Cell Reports 12, 370–374 (1993). https://doi.org/10.1007/BF00234695
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00234695