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Characterization of monoclonal antibodies identifying type and strain-specific epitopes of human immunodeficiency virus type 1

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Summary

Several hybridoma cell lines were raised against the highly cytopathic Zairian isolate of Human Immunodeficiency Virus (HIV), HIV1-NDK.

The specificity of the secreted monoclonal antibodies (mAb) was demonstrated by immunoblotting, radioimmunoprecipitation and immunofluorescence. Two hybridoma cell lines secreted mAb reacting with independent epitopes of the NDK p17 capsid protein and its precursors. One, RL16.24.5, is specific for the NDK isolate whereas the other, RL16.45.1, along with anti-p25 RL16.30.1 mAb, bind all HIV1 isolates but not HIV2. Together with the previously described mAb RL4.72.1 those reagents define lentivirus subfamily (HIV1, HIV2, SIV) type/subtype (HIV1) and strain (HIVI-NDK) specific epitopes expressed on HIVl-NDK core proteins. The last mAb RL16.76.1 binds the env gene products gp160 and gp120.

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Author notes

  1. Véronique Robert, Mariana Resnicoff & Christian Devaux

    Present address: Centre de tri des molécules anti-HIV de Montpellier, Montpellier, France

Authors and Affiliations

  1. INSERM U.322, Unité de Recherches sur les Rétrovirus et Maladies Associées, Campus de Luminy, BP 33, 13273, Marseille Cédex 9, France

    Véronique Robert, Mariana Resnicoff, Jean-Claude Chermann & Christian Devaux

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  1. Véronique Robert
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  2. Mariana Resnicoff
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  3. Jean-Claude Chermann
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Robert, V., Resnicoff, M., Chermann, JC. et al. Characterization of monoclonal antibodies identifying type and strain-specific epitopes of human immunodeficiency virus type 1. Mol Cell Biochem 102, 115–123 (1991). https://doi.org/10.1007/BF00234569

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  • DOI: https://doi.org/10.1007/BF00234569

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