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Influence of medium and cold pretreatment on androgenetic response in Lolium perenne L.

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Summary

Genotypes of Lolium perenne L. with different androgenetic responses were used to test effects of induction medium composition. The media tested were potato II (pII), 190-2, and modified Linsmaier and Skoog media, LS-1, LS-2, and LS-3. The effect of different gelling agents, activated charcoal in a ‘double layer’ design, and casein hydrolysate were also studied. From 36,696 anthers, 25,906 embryo-like structures, 1,959 albino and 173 green plants were generated. Significant differences were found between media, genotypes and medium-genotype interactions studied. All three media commonly used, pII, 190-2, and LS-3, were equivalent in production of green plants. Cold pretreatment of the anthers (4°C) significantly increased the number of embryo-like structures, the number and proportion of albino plants produced, but not the production of green plants.

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Abbreviations

ELS:

embryo-like structures

ALB:

albino plants

ANT:

anthers

GRP:

green plants

DH:

doubled haploid plants

AC:

activated charcoal

CH:

casein hydrolysate

2,4-D:

2,4-dichlorophenoxyacetic acid

LS:

Linsmaier and Skoog (1965) basal medium

MS:

Murashige and Skoog (1962)

PL:

plants

pII:

potato II induction medium

DL:

double layer

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Authors and Affiliations

  1. Department of Biotechnological Sciences, Agricultural University of Norway, POB 5040, N-1432, Ås, Norway

    Hilde-Gunn Opsahl-Ferstad & Odd Arne Rognli

  2. Department of Horticulture and Crop Sciences, Agricultural University of Norway, POB 5022, N-1432, Ås, Norway

    Åsmund Bjørnstad

Authors
  1. Hilde-Gunn Opsahl-Ferstad
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  2. Åsmund Bjørnstad
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  3. Odd Arne Rognli
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Communicated by K. Glimelius

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Opsahl-Ferstad, HG., Bjørnstad, Å. & Rognli, O.A. Influence of medium and cold pretreatment on androgenetic response in Lolium perenne L.. Plant Cell Reports 13, 594–600 (1994). https://doi.org/10.1007/BF00234518

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  • DOI: https://doi.org/10.1007/BF00234518

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