Summary
Embryogenic masses were obtained from immature leaves of peanut (Arachis hypogaea L.) cultured on a medium containing 20 mg/l 2,4-D. Somatic embryos developed from these masses following transfer to a medium containing 3 mg/l 2,4-D. The embryo morphology was quite variable. Following transfer to hormone-free medium, these embryos germinated. Shoot elongation was obtained in 25% of the embryos following transfer to a medium supplemented with 0.5 mg/l each of BAP and Kn. The plants grown in vitro by this method survived in sand:soil mixture and were grown to maturity.
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Abbreviations
- ABA:
-
abscisic acid
- BAP:
-
6-benzyl amino purine
- 2,4-D:
-
2,4 dichlorophenoxyacetic acid
- GA3 :
-
gibberellic acid
- Kn:
-
kinetin
- NAA:
-
1-naphthaleneacetic acid
- 2,4,5-T:
-
2,4,5-trichlorophenoxyacetic acid
- Z:
-
zeatin
References
Ammirato PV, (1987). In: Plant Tissue And Cell Culture (Green CD, Somer DA,Hackett WP, Biesboer DD eds) AR Liss Inc. 57–81.
Baker CM, Wetzstein HY (1992). Plant Cell Rep., 11:71–75.
Hazra S, Sathaye SS, Mascarenhas AF (1989). Biotechnology, 7: 949–951
Kerns HR, Barwale UB, Meyer MM Jr, Wildholm JM (1986). Plant Cell Rep., 5:140–143.
Murashige, T, Skoog F. (1962). Physiol. Plant., 15:473–497
Ozias-Akins P (1989). Plant Cell Rep., 8:217–218.
Parrott WA, Merkle SA, Williams EG (1991). Advanced Methods in Plant Breeding and Biotechnology (Murray DR ed) CAB Int. 158–200.
Ramdev Reddy L, Reddy GM (1993). Ind. J. Expt. Biol., 31:57–60.
Senaratna T (1992) Biotech. Adv., 10:379–392
Trigiano RN, Beaty RM, Graham ET (1988). Plant Cell Rep., 7:148–150.
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Communicated by J. J. Finer
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Chengalrayan, K., Sathaye, S.S. & Hazra, S. Somatic embryogenesis from mature embryo-derived leaflets of peanut (Arachis Hypogaea L.). Plant Cell Reports 13, 578–581 (1994). https://doi.org/10.1007/BF00234515
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DOI: https://doi.org/10.1007/BF00234515