Summary
Myosin was localized in rat intestinal epithelium by means of indirect immunofluorescence and immunoelectron microscopy (unlabeled antibody peroxidase method), using a specific antibody to myosin from chicken gizzard. Immunoreactivity was localized in the apical cytoplasm, where it was concentrated along the rootlets of the microvillar filament bundles and in the terminal web. A model of microvillar contraction is proposed.
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References
Begg, D.A., Rodewald, R., Rebhun, L.I.: The visualization of actin filament polarity in thin sections. Evidence for the uniform polarity of membrane-associated filaments. J. Cell Biol. 79, 846–852 (1978)
Bretscher, A., Weber, K.: Purification of microvilli and an analysis of the protein components of the microfilament core bundles. Exp. Cell. Res. 116, 397–407 (1978a)
Bretscher, A., Weber, K.: Localization of actin and microfilament-associated proteins in the microvilli and terminal web of the intestinal brush border by immunofluorescence microscopy. J. Cell Biol. 79, 839–645 (1978b)
Drenckhahn, D., Unsicker, K., Griesser, G.-H., Schumacher, U., Gröschel-Stewart, U.: Different myosins in myoid and entodermal reticular epithelial cells of the thymus. An immunocytochemical study using specific antibodies against striated and smooth muscle myosin. Cell Tissue Res. 187, 97–103 (1978)
Gröschel-Stewart, U., Schreiber, J., Mahlmeister, C., Weber, K.: Production of specific antibodies to contractile proteins, and their use in immunofluorescence microscopy. I. Antibodies to smooth and striated chicken muscle myosins. Histochemistry 46, 229–236 (1976)
Ishikawa, H., Bischoff, R., Holtzer, H.: Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J. Cell Biol. 43, 312–328 (1969)
Mooseker, M.S.: Brush border motility. Microvillar contraction in Triton-treated brush borders isolated from intestinal epithelium. J. Cell Biol. 71, 417–433 (1976)
Mooseker, M.S., Tilney, L.G.: Organization of an actin filament-membrane complex. Filament polarity and membrane attachment in the microvilli of intestinal epithelial cells. J. Cell Biol. 67, 725–743 (1975)
Mooseker, M.S., Pollard, T.S., Fujiwara, K.: Characterization and localization of myosin in the brush border of intestinal epithelial cells. J. Cell Biol. 79, 444–453 (1978)
Rodewald, R., Newman, S.B., Karnovski, M.J.: Contraction of isolated brush borders from the intestinal epithelium. J. Cell Biol. 70, 541–554 (1976)
Sandström, B.: A contribution to the concept of brush border function. Observations in intestinal epithelium in tissue culture. Cytobiologie 3, 293–297 (1971)
Thuneberg, L., Rostgaard, J.: Motility of microvilli. A film demonstration. J. Ultrastruct. Res. 29, 578 (1969)
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Drenckhahn, D., Steffens, R. & Gröschel-Stewart, U. Immunocytochemical localization of myosin in the brush border region of the intestinal epithelium. Cell Tissue Res. 205, 163–166 (1980). https://doi.org/10.1007/BF00234452
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DOI: https://doi.org/10.1007/BF00234452