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Scanning and transmission electron microscopic study of the lung of the newt, Triturus alpestris Laur.

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Summary

The lungs of Triturus alpestris Laur. were investigated with the scanning and transmission electron microscopes. Dimensions of the cell bodies of pneumocytes and ciliated cells, as well as the thickness of the air-blood barrier, were determined. The lungs of the newt form two simple sacs without septa. A ciliated epithelium containing goblet cells lines the pulmonary vein and partially the pulmonary artery. The remainder of the lung surface is covered internally by respiratory epithelium consisting of one type of cell and only occasionally showing the presence of single ciliated cells. All cells, ciliated, goblet and pneumocytes, contain in their cytoplasm lamellar bodies. Multivesicular bodies and numerous vesicles of variable electron density also occur in the cytoplasm of pneumocytes. Atypical mitochondria can be found in all cell types of the lung. Fixation with addition of tannic acid reveals the surface lining film. Tubular myelin figures were not observed.

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References

  • Callas, G., De Groot, W.J.: Lamellar transformation from type II pneumocyte mitochondria in rat lung. Anat. Rec. 184, 369 (1976)

    Google Scholar 

  • Czopek, J.: Quantitative studies on the morphology of respiratory surfaces in amphibians. Acta Anat. (Basel) 62, 296–323 (1965)

    Google Scholar 

  • Godula, J.: Intramitochondrial complexes of atypical structures in hepatocytes of Triturus alpestris (Laurenti). Experientia 28, 453–455 (1972)

    Google Scholar 

  • Goniakowska-Witalińska, L.: Ultrastructural and morphometric study of the lung of the European salamander Salamandra salamandra. Cell Tissue Res. 191, 343–356 (1978)

    Google Scholar 

  • Goniakowska-Witalinska, L.: Ultrastructural and morphometric changes in the lung of newt, Triturus cristatus cranifex Laur. during ontogeny. J. Anat. (1979) in press

  • Hall, J.D., Crane, F.L.: An intracristal structure in beef heart mitochondria. Exptl. Cell Res. 62, 480–483 (1970)

    Google Scholar 

  • Hanzlikova, V., Schiaffmo, S.: Mitochondrial changes in ischemic skeletal muscle. J. Ultrastruct. Res. 60, 121–133 (1977)

    Google Scholar 

  • Hightower, J.A., Burke, J.D., Haar, J.L.: A light and electron microscopic study of the respiratory epithelium of the adult aquatic newt, Notophthalmus viridescens. Can. J. Zool. 53, 465–472 (1975)

    Google Scholar 

  • Hughes, G.M.: Ultrastructure of the air-breathing organs of some lower vertebrates. 7e Congress Internat, de Microscopie Electronique 3, pp. 599–600. Grenoble, 1970

  • Hughes, G.M., Weibel, E.R.: Visualization of layers lining the lung of South American lungfish (Lepidosiren paradoxa) and a comparison with the frog and rat. Tissue and Cell 10, 343–353 (1978)

    Google Scholar 

  • Kalina, M., Pease, D.C.: The preservation of ultrastructure in saturated phosphatidyl cholines by tannic acid in model systems and type II pneumocytes. J. Cell Biol. 74, 726–741 (1977a)

    Google Scholar 

  • Kalina, M., Pease, D.C.: The probable role of phosphatidyl cholines in the tannic acid enhancement of cytomembrane electron contrast. J. Cell Biol. 74, 742–746 (1977b)

    Google Scholar 

  • Meban, C.: Ultrastructure of the respiratory epithelium in the lungs of the newt Triturus cristatus. Acta Zool. (Stockh.) 58, 151–156 (1977)

    Google Scholar 

  • Meban, C.: An electron microscope study of the respiratory epithelium in the lungs of the fire salamander (Salamandra salamandra). J. Anat. 128, 215–221 (1979)

    Google Scholar 

  • Newcomb, E.H., Steer, M.W., Hepler, P.K., Wergin, W.P.: An atypical crista resembling a “tight junction” in bean root mitochondria. J. Cell Biol. 39, 35–42 (1968)

    Google Scholar 

  • Noaillac-Depeyre, J., Gas, J.: Ultrastructural and cytochemical study of the gastric epithelium in a fresh water teleostean fish (Perca fluviatilis). Tissue and Cell 10, 23–37 (1978)

    Google Scholar 

  • Okada, Y., Ishiko, S., Daido, S., Kim, J., Ikeda, S.: Comparative morphology of the lung with special reference to the alveolar cells. I. Lung of the Amphibia. Acta Tuberculosea Japonica 11, 63–72 (1962)

    Google Scholar 

  • Pattle, R.E., Schock, C., Creasey, J.M., Hughes, G.M.: Surpellic films lung surfactant, and their cellular origin in newt, caecilian and frog. J. Zool. Lond. 182, 125–136 (1977)

    Google Scholar 

  • Perry, S.F.: Model of exchange barrier and respiratory surface area in the lung of the tortoise (Testudo graeca) and its practical application. Mikroskopie 32, 282–293 (1976)

    Google Scholar 

  • Richardson, K.C., Jarret, L.J., Finke, E.H.: Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Technol. 35, 313–323 (1960)

    Google Scholar 

  • Sorokin, S.P.: Histochemistry in embryonic lungs. In: Neonatal respiratory adaptation, pp. 79–93 (T.K. Oliver, Jr., ed.). U.S. Publ. Health Service, Publ. No. 1432 (1963)

  • Sorokin, S.P.: A morphological and cytochemical study on the great alveolar cell. J. Histochem. Cytochem. 14, 884–898 (1966)

    Google Scholar 

  • Sperry, D.G., Wassersug, R.J.: A proposed function for microridges on epithelial cells. Anat. Rec. 185, 253–258 (1976)

    Google Scholar 

  • Stratton, C.J.: The ultrastructure of multilamellar bodies and surfactant in the human lung. Cell Tissue Res. 193, 219–229 (1978)

    Google Scholar 

  • Takahashi, G.: OsO4-tannin-OsO4 method for transmission and scanning electron microscopy of biological specimens. 9th Internat. Congress on Electron Microscopy 2, pp. 56–57, Toronto (1978)

  • Valdivia, E., Berger, J.E.: Atypical electron microscope structures in liver mitochondria. Path. Microbiol. 39, 112–114 (1973)

    Google Scholar 

  • Weibel, E.R., Palade, G.E.: New cytoplasmic components in arterial endothelia. J. Cell Biol. 23, 101–112 (1964)

    Google Scholar 

  • Weibel, E.R.: Morphological basis of alveolar-capillary gas exchange. Physiol. Rev. 53, 419–495 (1973)

    Google Scholar 

  • Whitford, W.G., Hutchison, V.H.: Gas exchange in salamanders. Physiol. Zool. 38, 228–242 (1965)

    Google Scholar 

  • Willnow, I.: Die Lunge von Amphiuma means means. I. Mitteilung: Morphologie. Zool. Beiträge 10, 29–85 (1964)

    Google Scholar 

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Goniakowska-Witalińska, L. Scanning and transmission electron microscopic study of the lung of the newt, Triturus alpestris Laur.. Cell Tissue Res. 205, 133–145 (1980). https://doi.org/10.1007/BF00234449

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