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Protoplast culture in high molecular oxygen atmospheres

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Abstract

The effect of a molecular oxygen atmosphere on protoplast culture, compared with normal cultural procedures, was investigated for tomato, rice and jute protoplast systems. Protoplasts, in unsealed Petri dishes were cultured in a sealed 250 ml volume container in which the atmosphere was replaced with sterile molecular oxygen (O2 100% v/v) achieved by a one minute oxygen flow (10 mBar). Controls were cultured in a similar way but with a normal atmosphere (20.5% v/v oxygen). For all three species a major improvement in plating efficiency and colony formation was observed in the oxygen-enriched atmospheres. In the case of rice, cultivar Taipei 309, a significant increase in plant regeneration capacity, from protoplast-derived colonies, was observed for the high oxygen cultures.

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Abbreviations

MES:

2-N-morpholinoethane sulfonic acid

PE:

plating efficiency

Reference

  • Abdullah R, Thompson J.A. and Cocking E.G. (1986) Bio/Technology 4 (12), 1087–1090.

    Google Scholar 

  • Bellincampi D, Morpurgo G (1987) Plant Sci. 51: 83–91.

    Google Scholar 

  • Carman JG (1987) Planta 175: 417–424.

    Google Scholar 

  • Jackson MB, Abbott AJ, Belcher AR and Hall KC (1987) British Plant Growth Regulator Group, Monograph 16: 57–71.

    Google Scholar 

  • Kao KN and Michayluk MR (1975) Planta 126: 105–110.

    Google Scholar 

  • Kyozuka J, Hayashi Y and Shinomoto K. (1987) Mol. Gen. Genet. 206: 408–413.

    Google Scholar 

  • Lee L, Schroll RE, Grimes HD, Hodges TK (1990) Planta 178 (3): 325–333.

    Google Scholar 

  • Mei-Lei MCT, Reitveld EM, van Marrewijk GAM and Kool AJ (1987) Plant Cell Reports 6: 172–175.

    Google Scholar 

  • Power JB, McLellan M and Davey MR (1989) Laboratory Manual: Plant Tissue Culture, Dept. of Botany, University of Nottingham, U.K.

    Google Scholar 

  • Roest S and Gilissen LJW (1989). Acta Bot. Neerl. 38(1): 1–23.

    Google Scholar 

  • Shahin EA (1985) Theor. Appl. Genet., 69: 235–240.

    Google Scholar 

  • Shillito RD, Paszkowski J and Potrykus I (1983) Plant Cell Reports 2: 244–247.

    Google Scholar 

  • Van der Plas LHW and Wagner MJ (1986) Plant Cell Tiss. Org. Cult. 7: 217–225.

    Google Scholar 

  • Zhang, W. and Wu, R. (1988) Theor. Appl. Genet. 76: 835–840.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, NG7 2RD, Nottingham, UK

    E B. d'Utra Vaz, I. H. Slamet, A. Khatun, E. C. Cocking & J. B. Power

Authors
  1. E B. d'Utra Vaz
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  2. I. H. Slamet
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  3. A. Khatun
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  4. E. C. Cocking
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  5. J. B. Power
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Communicated by N. Amrhein

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Vaz, E.B.d., Slamet, I.H., Khatun, A. et al. Protoplast culture in high molecular oxygen atmospheres. Plant Cell Reports 11, 416–418 (1992). https://doi.org/10.1007/BF00234373

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  • DOI: https://doi.org/10.1007/BF00234373

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