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Plant regeneration from hypocotyls, cotyledons and stem segments of the C3–C4 intermediate species Moricandia arvensis

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Abstract

Techniques have been developed for the regeneration of Moricandia arvensis from complex explants. Hypocotyl segments and cotyledonary explants regenerated shoots, but the most efficient plant regeneration was from stem sections taken from in vitro grown shoots. Regeneration from these three explant types was tested on a range of concentrations of benzylaminopurine and either naphthylene acetic acid or indole acetic acid. Regeneration from all three explants was much higher on indole acetic acid than on naphthylene acetic acid and the ratio of auxin to cytokinin was also significant in determining the response of explants. Optimum regeneration was on 1mg/l IAA with 1mg/l BAP. Plants could be transferred to soil and grown to flowering in the glasshouse.

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Abbreviations

GDC:

glycine decarboxylase

BAP:

benzyl aminopurine

NAA:

naphthalene acetic acid

IAA:

indole acetic acid

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Author notes

  1. G. S. Khehra

    Present address: Department of Life Sciences, University of Nottingham, University Park, NG7 2RD, Nottingham, UK

Authors and Affiliations

  1. Brassica and Oilseed Research Department, Cambridge Laboratory, John Innes Centre, Colney Lane, NR4 7UJ, Norwich, UK

    G. S. Khehra & R. J. Mathias

Authors
  1. G. S. Khehra
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  2. R. J. Mathias
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Additional information

Communicated by M. R. Davey

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Khehra, G.S., Mathias, R.J. Plant regeneration from hypocotyls, cotyledons and stem segments of the C3–C4 intermediate species Moricandia arvensis . Plant Cell Reports 11, 412–415 (1992). https://doi.org/10.1007/BF00234372

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  • DOI: https://doi.org/10.1007/BF00234372

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