Skip to main content
SpringerLink
Log in

Plant regeneration from mesophyll protoplasts of Moricandia arvensis

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Moricandia arvensis is of interest as it is a dicotyledonous species which has C3 — C4 intermediate photosynthesis, a mechanism which results in enhanced recapture of photorespired CO2. Leaves from cultured shoot tips were used as a source for mesophyll cell protoplasts. Approximately 1% of the protoplasts which survived the first few days of culture produced calli. On a suitable regeneration medium, 30–60% of the calli regenerated one or more shoots. From among the regenerating shoots eight were selected, transferred to soil and grown to flowering in the glasshouse; all were fertile. The development of a protoplast regeneration system provides the opportunity to use transformation and somaclonal variation as tools in the genetic analysis of the C3–C4 character in this species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

GDC:

glycine decarboxylase

2,4-D:

2,4-dichlorophenoxyacetic acid

BAP:

benzyl aminopurine

NAA:

naphthalene acetic acid

ABA:

abscisic acid

References

  • Apel P, Bauwe H, Ohle H (1984) Biochem Physiol Pflanzen 179:793–797

    Google Scholar 

  • Banks MS, Evans PK (1976) Plant Sci Lett 7:409–416

    Google Scholar 

  • Holaday AS, Chollet R (1984) Photosynthesis. Res 5:307–323

    Google Scholar 

  • Holaday AS, Harrison AT, Chollet R (1982) Plant Sci Lett 27:181–189

    Google Scholar 

  • Holaday AS, Sheih Y-J, Lee KW, Chollet R (1981) Biochem Biophys Acta 637:334–341

    Google Scholar 

  • Kao KN, Michayluk MR (1975) Planta 126:105–110

    Google Scholar 

  • Manton I (1932) Ann Bot 49:509–556

    Google Scholar 

  • McVetty PBE, Austin RB, Morgan CL (1989) Ann Bot 64:87–94

    Google Scholar 

  • Menczel L, Nagy F, Kiss ZsR, Maliga P (1981) Theor Appl Gen 59:191–195

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Nagy JI, Maliga P (1976) Z Pflanzenphysiol 78:453–455

    Google Scholar 

  • Poulsen GB, Nielsen VS (1989) Plant Cell,Tissue and Organ Culture 17:153–158

    Google Scholar 

  • Rawsthorne S, Hylton CM, Smith AM, Woolhouse HW (1988) Planta 173:298–308

    Google Scholar 

  • Toriyama K, Hinata K, Kameya T (1987) Plant Sci 48:123–128

    Google Scholar 

  • Toriyama K, Kameya T, Hinata K (1987) Plant Tiss Cult Lett 4:75–78

    Google Scholar 

Download references

Author information

Author notes

  1. T. Murata

    Present address: Faculty of Agriculture, Kyushu Tokai University, Kawayo, Choyo-Son, Aso-gun, 869-14, Kumamoto, Japan

Authors and Affiliations

  1. Brassica and Oilseed Research Department, Cambridge Laboratory, John Innes Centre, Colney Lane, NR4 7UJ, Norwich, UK

    T. Murata & R. J. Mathias

Authors
  1. T. Murata
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. J. Mathias
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by M. R. Davey

Rights and permissions

Reprints and permissions

About this article

Cite this article

Murata, T., Mathias, R.J. Plant regeneration from mesophyll protoplasts of Moricandia arvensis . Plant Cell Reports 11, 408–411 (1992). https://doi.org/10.1007/BF00234371

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00234371

Keywords

Search

Navigation