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Regeneration of fertile plants from protoplasts of sunflower (Helianthus annuus L.)

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Summary

Sunflower hypocotyl protoplasts (Helianthus annuus L.) from 5 PIONEER genotypes (PT024, SMF3, EMIL, HA300*PT024, VK5F) and 1 public line (RHa 274) formed colonies at frequencies of up to 60% when plated in 0.25ml agarose beads in a modified L4 medium (Lenée and Chupeau 1986) containing 3mg/l NAA, 1mg/l BA and 0.1mg/l 2,4-D, and 1000mg/l casamino acids. Protoplast-derived colonies grew slowly into calli. Organogenesis was obtained from callus of PT024 on a MS medium containing NAA and BA at 1mg/l and GA at 0.1mg/l. Freshly excised shoots were induced to root by an IAA treatment. Regenerated plants were transferred to the greenhouse and seed was harvested within 7 months of the initial protoplast isolation.

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Abbreviations

BA:

6-benzylaminopurine

NAA:

α-naphtaleneacetic acid

GA:

gibberellic acid

IAA:

indole-3-acetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

MS:

Murashige and Skoog mineral elements

B5:

Gamborg mineral elements

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Authors and Affiliations

  1. Pioneer Hi-Bred International, Inc., Biotechnology Research, 7300 NW 62nd Avenue, 50131, Johnston, IA, USA

    Monique Burrus, Christel Chanabe & Gilbert Alibert

  2. Laboratoire de Biotechnologie et Amélioration des plantes ENSA, 145 Av. de Muret, 31076, Toulouse, France

    Monique Burrus & Dennis Bidney

Authors
  1. Monique Burrus
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  2. Christel Chanabe
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  3. Gilbert Alibert
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  4. Dennis Bidney
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Communicated by R. B. Horsch

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Burrus, M., Chanabe, C., Alibert, G. et al. Regeneration of fertile plants from protoplasts of sunflower (Helianthus annuus L.). Plant Cell Reports 10, 161–166 (1991). https://doi.org/10.1007/BF00234286

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  • DOI: https://doi.org/10.1007/BF00234286

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