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Ultrastructural localization of δ5-3β-hydroxysteroid dehydrogenase in the interrenal cells of the goldfisch (Carassius auratus)

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Summary

The ultrastructural localization of the enzyme Δ5-3β-hydroxysteroid dehydrogenase (3β-HSD) was studied in the goldfish interrenal cells by using the potassium ferricyanide method. Immersion fixation in a mixture of 1 % paraformaldehyde and 0.25% glutaraldehyde results in a consistently good ultrastructural preservation and enzyme localization. A precipitate of copper ferrocyanide indicating the localization of 3β-HSD activity was observed in close vicinity to the smooth endoplasmic reticulum or in contact with the outer surface of its membrane. A small number of precipitated grains were also found in the lumen of mitochondrial cristae. Addition of phenazine methosulfate increased the intensity of the reaction without changing the localization of the copper ferrocyanide grains. These findings suggest that the outer surface of the smooth endoplasmic reticulum is the major site of 3β-HSD activity in goldfish interrenal cells.

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References

  • Bara G (1968) Histochemical study of 3β-, 3α-, 11β-, and 17β-hydroxysteroid dehydrogenases in the adrenocortical tissue and the corpuscles of Stannius of Fundulus heteroclitus. Gen Comp Endorinol 10:126–137

    Google Scholar 

  • Berchtold JP (1977) Ultracytochemical demonstration and probable localization of 3β-hydroxysteroid dehydrogenase activity with a ferricyanide technique. Histochemistry 50:175–190

    Google Scholar 

  • Berchtold JP (1978) Electron microscopic localization of 3β-hydroxysteroid dehydrogenase and NADH-ferricyanide reductase activities in amphibian interrenal cells. Cell Tissue Res 188:143–148

    Google Scholar 

  • Berchtold JP (1979) Localisation ultrastructurale des activités 3β-hydroxystéroïde déshydrogénasique et NADH-ferricyanure réductasique dans les cellules du cortex surrénalien de rat. Biol Cellulaire 34:65–70

    Google Scholar 

  • Berchtold JP, Collenot G, Collenot A (1978) Localisation ultrastructurale de l'activité 3β-hydroxystéroïde déshydrogénasique dans les cellules du tissu glandulaire du testicule de Pleurodeles waltlii Michah (Amphibien, Urodèle). C R Acad Sci Paris 287:série D 511–513

    Google Scholar 

  • Beyer KF, Samuels LT (1956) Distribution of steroid 3β-ol-dehydrogenase in cellular structures of the adrenal gland. J Biol Chem 219:69–76

    Google Scholar 

  • Bhattacharyya TK, Butler DG (1979) Fine structure of the adrenocortical homologue in the North American eel and modifications following seawater adaptation. Anat Rec 193:213–232

    Google Scholar 

  • Guraya SS (1976) Recent advances in the morphology, histochemistry, biochemistry of steroid-synthesizing cellular sites in the nonmammalian vertebrate ovary. Int Rev Cytol 44:365–409

    Google Scholar 

  • Hanke W, Chester Jones I (1966) Histological and histochemical studies on the adrenal cortex and the corpuscles of Stannius of the European eel (Anguilla anguilla L.). Gen Comp Endocrinol 7:166–178

    Google Scholar 

  • Hoar WS (1969) Reproduction. In: Hoar WS, Randall DJ (eds) Fish Physiology. Vol. 3. Academic Press, New York

    Google Scholar 

  • Iwasaki Y (1973) Histochemical detection of Δ5-3β-hydroxysteroid dehydrogenase in the ovary of Medaka, Oryzias latipes, during annual reproductive cycle. Bull Fac Fish Hokkaido Univ 23:177–184

    Google Scholar 

  • Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol 27:137A

    Google Scholar 

  • Kream BE, Sauer LA (1976) On the intracellular localization of the 3β-hydroxysteroid dehydrogenase/isomerase in rat adrenal cortex. Proc Soc Exp Biol Med 152:459–462

    Google Scholar 

  • Lambert JGD, van Oordt PGWJ (1974) Ovarium hormones in teleosts. Fortschr Zool 22:340–349

    Google Scholar 

  • Lofts B, Bern HA (1972) The functional morphology of steroidogenic tissues. In: Idler DR (ed) Steroids in Nonmammalian Vertebrates. Academic Press, New York, pp 37–126

    Google Scholar 

  • Ogawa M (1967) Fine structure of the corpuscles of Stannius and the interrenal tissue in goldfish, Carassius auratus. Z Zellforsch 81:174–189

    Google Scholar 

  • Yamamoto K, Onozato H (1965) The fine structure of the interrenal tissue of the goldfish. Annot Zool Jpn 38:140–150

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Reproductive Biology, National Institute for Basic Biology, 444, Okazaki, Japan

    H. Kagawa &  Y. Nagahama

  2. Department of Biology, Faculty of Fisheries, Hokkaido University, 041, Hakodate, Japan

    H. Kagawa

Authors
  1. H. Kagawa
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  2. Y. Nagahama
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Kagawa, H., Nagahama, Y. Ultrastructural localization of δ5-3β-hydroxysteroid dehydrogenase in the interrenal cells of the goldfisch (Carassius auratus). Cell Tissue Res. 212, 225–231 (1980). https://doi.org/10.1007/BF00233957

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  • DOI: https://doi.org/10.1007/BF00233957

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