Summary
Normal quiescent lymphocytes regulate their ribosome content by selectively degrading newly synthesized 18S ribosomal RNA. Unlike actively dividing HeLa cells, lymphocytes retain 18 S ribosomal RNA in the nucleus after synthesis instead of immediately transporting it to the cytoplasm. Subcellular fractionation of the highly differentiated human neoplastic lymphocyte RPMI-8226 reveals that this cell line also retains 18 S ribosomal RNA in the nucleus, a trait not displayed by the less differentiated human lymphoblastoid cell line RPMI-4265. These observations suggest that neoplastic cells can be phenotypically characterized by their ribosomal RNA processing patterns.
This is a preview of subscription content, log in via an institution to check access.
References
Adelman, M.R.C., Blobel, G., Sabatini, D.D.: An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes. J. Cell Biol. 56, 191–205 (1973)
Buckton, K.E., Court-Brown, W.M., Smith, P.G.: Lymphocyte survival in men treated with X-ray for ankylosing spondylitis. Nature 214, 470–473 (1973)
Bynum, J.W., Regan, J.D., Volkin, E.: RNA synthesis and processing as a measure of phenotypic variability in cytodifferentiation and neoplasia. J. Cell Physiol. 91, 1–14 (1977)
Bynum, J.W., Volkin, E.: Wasting of 18S ribosomal RNA by human myeloma cells cultured in adenosine. J. Cell. Physiol. 88, 197–206 (1976)
Cooper, J.L.: Ribosomal ribonucleic acid production and growth regulation in human lymphocytes. J. Biol. Chem. 244, 1947–1952 (1969)
Cooper, J.L., Kay, J.E.: Differential extraction of nuclear and cytoplasmic RNA from intact lymphocytes. Biochem. Biophys. Acta 174, 503–512 (1969)
Cooper, J.L., Rubin, A.D.: RNA metabolism in lymphocytes stimulated by phytohemagglutinin: Initial response to phytohemagglutinin. Blood 25, 1014–1027 (1965)
Craig, N.D., Perry, R.P.: Aberrant intranucleolar maturation of ribosomal precursors in the absence of protein synthesis. J. Cell. Biol. 45, 554–564 (1970)
Ennis, H.L.: Synthesis of ribonucleic acid in L cells during inhibition of protein synthesis by cycloheximide. Mol. Pharmacol. 2, 543–557 (1966)
Epstein, L.B., Stohman, F.Fr.: RNA synthesis in cultures of normal human peripheral blood. Blood 24, 69–75 (1964)
Norman, A., Sasaki, M.S., Ottoman, R.E., Fingerhut, A.G.: Lymphocyte lifetime in women. Science 147, 745 (1965)
Nowell, P.C.: Phytohemagglutinin: An initiator of mitosis in cultures, of normal human leukocytes. Cancer Res. 20, 462–466 (1960)
Nowell, P.C.: Unstable chromosome changes in tuberculin-stimulated leukocyte cultures from irradiated patients. Evidence for immunologically committed, long-lived lymphocytes in human blood. Blood 26, 798–804 (1965)
Penman, S.: RNA metabolism in the HeLa cell nucleus. J. Mol. Biol. 17, 117–130 (1966)
Penman, S.: Preparation of purified nuclei and nucleoli from mammalian cells. In: Fundamental Techniques in Biology. (K. Habel and N.P. Salzman, eds.) New York: Academic Press, pp. 35–48 (1969)
Pogo, B.G.T., Allfrey, V.G., Mirsky, A.E.: RNA synthesis and histone acetylation during the course of gene activation in lymphocytes. Proc. Nat. Acad. Sci. (USA) 55, 805–812 (1966)
Robinson, S.H., Brecher, G., Lourie, S.I. Hale, J.E.: Leukocyte labeling in rats during and after continuous infusion of tritiated thymidine: Implications for lymphocyte longevity and DNA reutilization. Blood 26, 281–295 (1965)
Takahashi, M., Yagi, Y., Moore, G.E., Pressman, D.: Pattern of immunoglobulin production in individual cells of human hematopoietic origin in established culture. J. Immunol. 102, 1274–1283 (1969)
Traub, A., Kaufmann, E., Ginsberg-Tietz, Y.: Studies on nuclear ribosomes. I. Association of DPN-pyrophosphorylase with nuclear ribosomes in normal and neoplastic tissues. Exptl. Cell Res. 34, 371–383 (1964)
Vaughan, M.H., Soeiro, R., Warner, J.R., Darnell, J.E.: The effect of methionine deprivation of ribosome synthesis in HeLa cells. Proc. Nat. Acad. Sci. (USA) 58, 1527–1534 (1967)
Wakefield, J.D., Thornbecker, G.J., Old, L.J., Boyse, E.A.: Production of immunoglobulins and their subunits by human tissue culture cell lines. J. Immunol. 99, 308–319 (1967)
Author information
Authors and Affiliations
Additional information
Operated by Union Carbide Corporation with the Department of Energy
Rights and permissions
About this article
Cite this article
Bynum, J.W., Volkin, E. Nuclear retention of 18S ribosomal RNA by human myeloma cells. Cell Tissue Res. 197, 347–351 (1979). https://doi.org/10.1007/BF00233925
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00233925