Abstract
Bulblet and callus cultures of Lilium testaceum were initiated in vitro from bulbscales. Continous propagation of the bulblet cultures was achieved on a modified Murashige and Skoog agar medium containing 1-naphthalene acetic acid (0.1 mg/l) and kinetin (0.1 mg/l) as phytohormones. The in vitro grown bulbs synthesized large quantities of storage ß-1,4-glucomannans (mannose: glucose = 7∶3; molecular weight = 200 kd) with an identical structure to the glucomannans from the in vivo grown bulbs. Higher 1-naphthalene acetic acid concentrations (1 mg/l) resulted in increased callus formation. Liquid suspension cultures derived from callus exhibited only small amounts of reserve glucomannans.
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Abbreviations
- DEAE:
-
2-(diethylamino)ethyl
- GF:
-
growth factor
- GM:
-
glucomannan
- GPC:
-
gel permeation chromatography
- IAA:
-
indole-3-acetic acid
- IEC:
-
ion exchange chromatography
- MS:
-
Murashige and Skoog
- MW:
-
molecular weight
- MWCO:
-
molecular weight cut off
- NAA:
-
1-naphthalene acetic acid
- NMR:
-
nuclear magnetic resonance
- PVP:
-
polyvinylpyrrolidone
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Communicated by P. Matrile
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Wozniewski, T., Blaschek, W. & Franz, G. In vitro propagation of Lilium testaceum and structural investigation of the storage β-1,4-glucomannan. Plant Cell Reports 10, 457–460 (1991). https://doi.org/10.1007/BF00233814
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DOI: https://doi.org/10.1007/BF00233814