Abstract
We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were evaluated. Callus produced on the Murashige and Skoog (MS) (1962) medium containing 2,4-D had low embryogenic potency. In the presence of 30 μM dicamba, addition of 2.25 to 9 μM BA significantly enhanced embryogenic callus formation over dicamba alone. Optimum frequency of somatic embryogenesis was achieved on MS basal medium containing 30 μM dicamba and 2.25 μM BA. Over 80% of somatic embryos germinated and formed plantlets on half-strength MS basal medium. These plantlets grew normally in the greenhouse.
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Abbreviations
- MS:
-
Murashige and Skoog medium
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- BA:
-
6-benzyladenine
- dicamba:
-
3, 6-dichloro-o-anisic acid
References
Ahn BJ, Huang FH, King JW (1985) Crop Sci. 25: 1107–1109
Artunduaga IR, Taliaferro CM, Johnson BB (1989) In vitro Cellular & Developmental Biology 25: 753–756
Bhaskaran S, Smith RH (1990) Crop Sci. 30: 1328–1336
Beard JB (1982) Turf management for golf courses Brahms G (ed) Burgerss Publishing Co. Minneapolis, Minnesota
Blanche FE, Krans JV, Coats GE (1986) Crop Sci. 26: 1245–1248
Cobb BG, Vanderzee D, Loescher WH, Kennedy RA (1985) Plant Science 40: 121–127
Conger BV, Hanning GE, Gray DJ, McDaniel JK (1983) Science 221: 850–851
Hanna WW, Lu C, Vasil IK (1984) Theor. Appl. Genet. 67: 155–159
Krans JV, Henning VT, Torres KC (1981) Crop Sci. 22: 1193–1197
Linacero R, Vazquez AM (1990) Plant Sci. 72: 253–258
Lu C, Vasil IK (1981) Theor. Appl. Genet. 59: 275–280
McDaniel RE, Conger BV (1984) Crop Sci. 24: 573–57
Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497
Raghavan V (1986) Embryogenesis in Angiosperms — A developmental and Experimental Study. Cambridge Univ. Press, Cambridge pp 303
Robert GDS, James HT (1980) Principles and procedures of statistics. McGraw-Hill Book Co. N.Y.
Trigiano RN, Gray DJ, Conger BV, McDaniel JK (1989) Bot. Gaz. 150: 72–77
Vasil V, Vasil IK (1981) Am. J. Bot. 68: 864–872
Vasil IK (1987) J. Plant Physiol. 128: 193–218
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Communicated by J.K. Vasil
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Zhong, H., Srinivasan, C. & Sticklen, M.B. Plant regeneration via somatic embryogenesis in creeping bentgrass (Agrostis palustris Huds.). Plant Cell Reports 10, 453–456 (1991). https://doi.org/10.1007/BF00233813
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DOI: https://doi.org/10.1007/BF00233813