Abstract
Novel rapid cycling Brassica napus lines have been produced by protoplast fusion between rapid cycling B. oleracea and rapid cycling B. rapa. Fusion products were selected based on iodoacetate inactivation and regeneration ability. A total of 36 plants was recovered from 3 regenerating calli. All were confirmed as somatic hybrids by morphological features, flow cytometric estimation of nuclear DNA content, RAPD analysis and/or DNA hybridization. Plants from two of the calli contained chloroplasts from B. rapa, and plants from the third contained B. oleracea chloroplasts. Some plants flowered in vitro, but on average flowering was initiated 22 days after transfer to soil. Although seed set was fairly low after self pollination, more seeds were obtained from pollination of open flowers than from pollination of buds. Seeds of the somatic hybrid B. napus showed novel fatty acid compositions, different from the mean of the two parental lines. Flowering was monitored in plants grown from seeds of the somatic hybrids, rapid cycling B. napus (CrGC 5-1) and the two diploid parental genotypes. Progeny of the somatic hybrids flowered faster and were more vigorous than rapid cycling B. napus (CrGC 5-1). The improved lines contain chloroplasts from B. rapa, unlike rapid cycling B. napus (CrGC 5-1), which has B. oleracea chloroplasts. The somatic hybrid lines produced may be useful for genetic studies or further in vitro manipulations.
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Abbreviations
- CrGC:
-
Crucifer Genetics Cooperative, University of Wisconsin-Madison
- MES:
-
1-morpholino-ethane sulfonate
- MS-3,0:
-
Murashige and Skoog medium containing 3% sucrose and no growth regulators
- RAPD:
-
random amplified polymorphic DNA
- RC:
-
rapid cycling
- RFLP:
-
restriction fragment length polymorphism
- std:
-
standard deviation
- TE:
-
10mM Tris, 1 mM EDTA, pH 8
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Hansen, L.N., Earle, E.D. Novel flowering and fatty acid characters in rapid cycling Brassica napus L. resynthesized by protoplast fusion. Plant Cell Reports 14, 151–156 (1994). https://doi.org/10.1007/BF00233780
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DOI: https://doi.org/10.1007/BF00233780