Summary
Simple one step micropropagation system for Cephaelis ipecacuanha A. Richard was developed using root cultures grown in vitro. Adventitious shoots were directly formed on the cut end of root segments without callus formation, on phytohormone-free B5 solid medium in the dark. When the shoots attached with root segments were further cultured under 16 h light / 8 h dark, they developed into plantlets, which could be transplanted to soil. The regenerated plants grew well in a greenhouse with showing normal appearance and accumulated alkaloids. The influence of auxin on adventitious shoot formation was also investigated.
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Abbreviations
- MS:
-
Murashige-Skoog (Murashige and Skoog 1962)
- 1/2 MS:
-
half strength MS
- B5:
-
Gamborg B5(Gamborg et al. 1968)
- WP:
-
woody plant (Lloyd and McCowm 1980)
- RC:
-
root culture (Thomas and Davey 1982)
- HF:
-
phytohormone free
- IAA:
-
indole-3-acetic acid
- NAA:
-
1-naphtaleneacetic acid
- TIBA:
-
2,3,5-triiodobenzoic acid
- 2,4,6-T:
-
2,4,6-trichlorophenoxyacetic acid
- SEM:
-
scanning electron microscopy
- C.V.:
-
coefficient of variation
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Communicated by A. Komamine
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Yoshimatsu, K., Shimomura, K. Plant regeneration on cultured root segments of Cephaelis ipecacuanha A. Richard. Plant Cell Reports 14, 98–101 (1994). https://doi.org/10.1007/BF00233769
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DOI: https://doi.org/10.1007/BF00233769