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Immunocytochemical localisation of prolactin and growth hormone in the perinatal sheep pituitary

A morphological and quantitative study

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Summary

The peroxidase anti-peroxidase immunocytochemical staining technique has been used to identify prolactin and growth hormone cells in pituitaries from fetal and neonatal sheep. The size of the secretory granules in these cell types has been measured using the image analysing computer Quantimet 720. The area size distributions of the fetal prolactin and growth hormone granules were compared with those in the neonate and the adult. It appears that the gestational age of the fetus may influence the size range of prolactin secretory granules.

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Author notes

  1. G. D. Thorburn

    Present address: Dept. of Physiology, University of Queensland, 4067, St. Lucia, Brisbane, Queensland, Australia

Authors and Affiliations

  1. Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, University of Oxford, OX3 9DU, Headington, Oxford, England

    D. M. Parry, I. C. McMillen, J. S. Robinson & G. D. Thorburn

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  1. D. M. Parry
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  2. I. C. McMillen
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  3. J. S. Robinson
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  4. G. D. Thorburn
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Parry, D.M., McMillen, I.C., Robinson, J.S. et al. Immunocytochemical localisation of prolactin and growth hormone in the perinatal sheep pituitary. Cell Tissue Res. 197, 501–514 (1979). https://doi.org/10.1007/BF00233573

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  • DOI: https://doi.org/10.1007/BF00233573

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