Abstract
The effect of thyroid hormones on the steadystate fluorescence polarization and on the release of the liposomal content was analyzed in liposomes composed of egg phosphatidylcholine and egg phosphatidyl choline: cholesterol in different molar ratios. Depending on liposome cholesterol composition, a dual effect of triiodothyronine was found. The fluorescence polarization of 1,6 diphenyl 1,3,5 hexatriene or 1-(4-trimethylaminophenyl) 6 phenyl-1, 3, 5 hexatriene decreased by the addition of the hormone when cholesterol content was in the range from 0 to 30 moles %, while it increased with cholesterol from 30 to 50 moles %. In the release experiments, the effect of triiodothyronine was also biphasie; the leakage was the highest at 0% and 50% and the lowest at 30 moles % of cholesterol. On the contrary, thyroxine was without effect on liposomes containing cholesterol from 30 to 50 mol %. This fact correlated with a lower incorporation of thyroxine, compared with that of triiodothyronine in liposomes containing up to 30 moles % of cholesterol.
The fact that the above differential incorporation of thyroid hormones was also observed at physiological concentration and that most of the mammalian membrane cells have more than 25 moles % of cholesterol have for physiological implications to the observations reported here.
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This work was supported by Grants PID 3-013800/89 from Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET), Fundación Antorchas A-12576/1-000065 and Consejo de Investigaciones de la Universidad Nacional de Tucumán (CIUNT). This paper was prepared during a sabbatical for RNF in Rome under a joint program with Argentine CONICET and Italian CNR, and in Bilbao, under a Visiting Professor program from the University of the Basque Country, Spain. We thank Dr. Raúl Salomón for assistance with the English manuscript.
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Chehin, R.N., Rintoul, M.R., Morero, R.D. et al. Differential effect of triiodothyronine and thyroxine on liposomes containing cholesterol: Physiological speculations. J. Membarin Biol. 147, 217–221 (1995). https://doi.org/10.1007/BF00233549
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DOI: https://doi.org/10.1007/BF00233549