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Putative sites of cytokinin action during their enhancing effect on indole alkaloid accumulation in periwinkle cell suspensions

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Summary

The effects of cytokinins on the different branches of the indole alkaloid pathway were investigated in Catharanthus roseus cell cultures. Addition of zeatin to a 2,4-dichlorophenoxyacetic acid-containing medium decreased tryptamine levels and increased the bioconversion of secologanin to ajmalicine. Zeatin also enhanced the geraniol-10 hydroxylase activities and modified the indole alkaloid pattern. The results are discussed in the light of previous works showing that cytokinins have a positive effect on indole alkaloid accumulation in some lines of C. roseus.

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Abbreviations

BSTFA:

bis(trimethylsilyl) trifluoroacetamide

CK:

cytokinin

2,4-D:

2,4-dichlorophenoxyacetic acid

dw:

dry weight

G-10H:

geraniol-10 hydroxylase

NAA:

αnaphthaleneacetic acid

SE:

standard error

TDC:

tryptophan decarboxylase

Z:

zeatin

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Authors and Affiliations

  1. Laboratoire de Biologie Cellulaire et Biochimie Végétale, Faculté de Pharmacie, Université de Tours, 37200, Tours, France

    A. Decendit, G. Petit, F. Andreu, J. M. Mérillon & M. Rideau

  2. Laboratoire de Physiologie Végétale, Faculté des Sciences, Université de Tours, France

    P. Doireau

Authors
  1. A. Decendit
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  2. G. Petit
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  3. F. Andreu
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  4. P. Doireau
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  5. J. M. Mérillon
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  6. M. Rideau
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Communicated by A. M. Boudet

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Decendit, A., Petit, G., Andreu, F. et al. Putative sites of cytokinin action during their enhancing effect on indole alkaloid accumulation in periwinkle cell suspensions. Plant Cell Reports 12, 710–712 (1993). https://doi.org/10.1007/BF00233425

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  • DOI: https://doi.org/10.1007/BF00233425

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