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The promoter of barley trypsin-inhibitor BTI-CMe, discriminates between wheat and barley endosperm protoplasts in transient expression assays

Abstract

Several promoter fragments from the barley gene coding for trypsin inhibitor, BTI-CMe, have been fused to the β-glucuronidase (GUS) reporter gene and these chimeric constructs used for transient expression in protoplasts. Transfection of developing endosperm protoplasts from barley (cv Bomi) show a maximum GUS expression of about 50% of that driven by the cauliflower mosaic virus 35S promoter, while in wheat endosperm protoplasts expression is less than 10%. No significant expression is found in transfected leaf protoplasts from barley, wheat or tobacco (<2% of the 35S control). All the information required for endosperm and barley specificity is present in the 343 bp proximal to the translation initiation site.

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Abbreviations

MS:

Murashige and Skoog medium

PEG:

polyethyleneglycol

GUS:

β-glucuronidase

MU:

methylumbelliferone

MUG:

4-methylumbelliferyl-β-D glucuronide

pp:

protoplasts

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Communicated by I. Potrykus

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Díaz, I., Royo, J. & Carbonero, P. The promoter of barley trypsin-inhibitor BTI-CMe, discriminates between wheat and barley endosperm protoplasts in transient expression assays. Plant Cell Reports 12, 698–701 (1993). https://doi.org/10.1007/BF00233422

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  • DOI: https://doi.org/10.1007/BF00233422

Keywords

  • Transient Expression
  • Trypsin Inhibitor
  • Promoter Fragment
  • Cauliflower Mosaic Virus
  • Translation Initiation Site