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The promoter of barley trypsin-inhibitor BTI-CMe, discriminates between wheat and barley endosperm protoplasts in transient expression assays


Several promoter fragments from the barley gene coding for trypsin inhibitor, BTI-CMe, have been fused to the β-glucuronidase (GUS) reporter gene and these chimeric constructs used for transient expression in protoplasts. Transfection of developing endosperm protoplasts from barley (cv Bomi) show a maximum GUS expression of about 50% of that driven by the cauliflower mosaic virus 35S promoter, while in wheat endosperm protoplasts expression is less than 10%. No significant expression is found in transfected leaf protoplasts from barley, wheat or tobacco (<2% of the 35S control). All the information required for endosperm and barley specificity is present in the 343 bp proximal to the translation initiation site.

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Murashige and Skoog medium








4-methylumbelliferyl-β-D glucuronide




  • Barber D, Sanchez-Monge R, Mendez E, Lazaro A, García-Olmedo F, Salcedo G (1986) Biochim Biophys Acta 869:115–118

    Google Scholar 

  • Carbonaro P, Salcedo G, Sanchez-Monge R, García-Maroto F, Royo J, Gomez L, Mena M, Medina J, Diaz I (1993) In: Aviles FX (ed) Innovations on proteases and their inhibitors. Walter de Gruyter, Berlin, New York (in press)

    Google Scholar 

  • Chong GL, Reeck GR (1987) Thrombosis Res 48:211–221

    Google Scholar 

  • Diaz I, Carbonero P (1992) Plant Cell Rep 10:595–598

    Google Scholar 

  • Gaff DF, Okomg'O-ogola O (1971) J Exp Bot 22:756–758

    Google Scholar 

  • García-Olmedo F, Salcedo G, Sanchez-Monge R, Hernandez-Lucas C, Carmona MJ, Lopez-Fando JJ, Fernandez JA, Gomez L, Royo J, GarcíaMaroto F, Castagnaro A, Carbonero P (1992) In: Shewry PR (ed) Barley, genetics, biochemistry, molecular biology and biotecnology. CAB International pp 335–350

  • Hauptman RM, Ozias-Aking P, Vasil V, Tabeizadesh Z, Rogers SG, Horsch RB, Vasil IK, Fraley RT (1987) Plant Cell Rep 6:265–270

    Google Scholar 

  • Jefferson RA (1987) Plant Mol Biol Rep 5:387–405

    CAS  Google Scholar 

  • Lee BT, Murdoch K, Topping J, Jones MGK, Kreis M (1991) Plant Sci 78:237–246

    Google Scholar 

  • Mahoney WX, Hermodson MA, Jomes B, Powers DD, Corfman RS, Reeck GR (1984) J Biol Chem 359:8412–8516

    Google Scholar 

  • Mikola J, Soulinna EM (1969) Eur J Biochem 9:555–560

    Google Scholar 

  • Negrutiu I, Shillato R, Potrykus I, Biasini G, Sala F (1987) Plant Mol Biol 8:363–373

    Google Scholar 

  • Power JB, Chapman JV (1985) In: Dixon RA (ed) Plant Cell Culture. IRL Press, Oxford, Washington DC pp 37–66

    Google Scholar 

  • Power JB, Davey MR, McLellan M, Wilson D (1989) Laboratory Mannual plant tissue culture. University of Nottingham, UK

    Google Scholar 

  • Rodriguez-Palenzuela P, Royo J, Gomez L, Sanchez-Monge R, Salcedo G, Molina-Cano JL, García-Olmedo F, Carbonero P (1989) Mol Gen Genet 219:474–479

    Google Scholar 

  • Royo J (1991) Doctoral Thesis. ETS Ingenieros Agrónomos. Universidad Politécnica de Madrid, Spain

    Google Scholar 

  • Teeri TH, Patel GK, Aspegren K, Kauppinen V (1989) Plant Cell Rep 8:187–190

    Google Scholar 

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Communicated by I. Potrykus

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Díaz, I., Royo, J. & Carbonero, P. The promoter of barley trypsin-inhibitor BTI-CMe, discriminates between wheat and barley endosperm protoplasts in transient expression assays. Plant Cell Reports 12, 698–701 (1993).

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  • Transient Expression
  • Trypsin Inhibitor
  • Promoter Fragment
  • Cauliflower Mosaic Virus
  • Translation Initiation Site