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The promoter of barley trypsin-inhibitor BTI-CMe, discriminates between wheat and barley endosperm protoplasts in transient expression assays

Plant Cell Reports volume 12, pages 698–701 (1993)Cite this article

Abstract

Several promoter fragments from the barley gene coding for trypsin inhibitor, BTI-CMe, have been fused to the β-glucuronidase (GUS) reporter gene and these chimeric constructs used for transient expression in protoplasts. Transfection of developing endosperm protoplasts from barley (cv Bomi) show a maximum GUS expression of about 50% of that driven by the cauliflower mosaic virus 35S promoter, while in wheat endosperm protoplasts expression is less than 10%. No significant expression is found in transfected leaf protoplasts from barley, wheat or tobacco (<2% of the 35S control). All the information required for endosperm and barley specificity is present in the 343 bp proximal to the translation initiation site.

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Abbreviations

MS:

Murashige and Skoog medium

PEG:

polyethyleneglycol

GUS:

β-glucuronidase

MU:

methylumbelliferone

MUG:

4-methylumbelliferyl-β-D glucuronide

pp:

protoplasts

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Authors and Affiliations

  1. Centro Nacional de Biotecnología, CSIC, 28049, Madrid, Spain

    Isabel Díaz & Pilar Carbonero

  2. Bioquímica y Biología Molecular, E.T.S.I. Agrónomos, Universidad Ploitécnica de Madrid, 28040, Madrid, Spain

    Joaquin Royo & Pilar Carbonero

Authors
  1. Isabel Díaz
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  2. Joaquin Royo
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  3. Pilar Carbonero
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Communicated by I. Potrykus

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Díaz, I., Royo, J. & Carbonero, P. The promoter of barley trypsin-inhibitor BTI-CMe, discriminates between wheat and barley endosperm protoplasts in transient expression assays. Plant Cell Reports 12, 698–701 (1993). https://doi.org/10.1007/BF00233422

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  • DOI: https://doi.org/10.1007/BF00233422

Keywords

  • Transient Expression
  • Trypsin Inhibitor
  • Promoter Fragment
  • Cauliflower Mosaic Virus
  • Translation Initiation Site