Abstract
Several promoter fragments from the barley gene coding for trypsin inhibitor, BTI-CMe, have been fused to the β-glucuronidase (GUS) reporter gene and these chimeric constructs used for transient expression in protoplasts. Transfection of developing endosperm protoplasts from barley (cv Bomi) show a maximum GUS expression of about 50% of that driven by the cauliflower mosaic virus 35S promoter, while in wheat endosperm protoplasts expression is less than 10%. No significant expression is found in transfected leaf protoplasts from barley, wheat or tobacco (<2% of the 35S control). All the information required for endosperm and barley specificity is present in the 343 bp proximal to the translation initiation site.
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Abbreviations
- MS:
-
Murashige and Skoog medium
- PEG:
-
polyethyleneglycol
- GUS:
-
β-glucuronidase
- MU:
-
methylumbelliferone
- MUG:
-
4-methylumbelliferyl-β-D glucuronide
- pp:
-
protoplasts
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Díaz, I., Royo, J. & Carbonero, P. The promoter of barley trypsin-inhibitor BTI-CMe, discriminates between wheat and barley endosperm protoplasts in transient expression assays. Plant Cell Reports 12, 698–701 (1993). https://doi.org/10.1007/BF00233422
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DOI: https://doi.org/10.1007/BF00233422