Skip to main content
SpringerLink
Log in

Effects of tissue type and promoter strength on transient GUS expression in sugarcane following particle bombardment

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Effects of tissue type and promoter strength on transient GUS expression in the sugarcane (Saccharum spp. hybrids) cultivar NCo 310 were evaluated following microprojectile bombardment of leaf explants. GUS expression was histochemically or fluorometrically measured 48 h after delivery of the uidA gene. High levels of GUS expression were obtained in leaf segments isolated from young, expanding sugarcane leaves cultured for 1, 3, or 6 d prior to bombardment. The promoter derived from the maize ubiquitin 1 gene (Ubi-1) produced significantly more GUS foci and higher GUS activity levels compared to the recombinant Emu, rice actin 1 (Act1), and CaMV 35S promoters. Our transient expression system should facilitate efforts to identify promoters and elements which will regulate desired gene expression patterns in sugarcane and aid in development of an efficient stable transformation system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

Act1 :

rice actin 1 gene

CaMV:

cauliflower mosaic virus

GUS:

ß-glucuronidase

Ubi-1:

maize ubiquitin 1 gene

uidA :

GUS gene

X-Glu:

5-bromo-4-chloro-3-indoylglucuronide

References

  • Battraw M, Hall TC (1991) Theor Appl Genet 82:161–168

    Google Scholar 

  • Bower R, Birch RG (1992) Plant J 2:409–416

    CAS  Google Scholar 

  • Chan M-T, Lee T-M, Chang H-H (1992) Plant Cell Physiol 33:577–583

    Google Scholar 

  • Chen WH, Gartland KMA, Davey MR, Sotak R, Gartland JS, Mulligan BJ, Power JB, Cocking EC (1987) Plant Cell Rep 6:297–301

    Google Scholar 

  • Chowdhury MKU, Vasil IK (1992) Plant Cell Rep 11:494–498

    Google Scholar 

  • Christensen AH, Sharrock RA, Quail PH (1992) Plant Mol Biol 18:675–689

    CAS  PubMed  Google Scholar 

  • Christou P, Ford TL, Kofron M (1991) Bio/Technol 9:957–962

    Google Scholar 

  • Dekeyser RA, Claes B, De Rycke RMU, Habets ME, Van Montagu MC, Caplan AB (1990) Plant Cell 2:591–602

    Google Scholar 

  • D'Halluin K, Bonne E, Bossut M, De Beuckeleer M, Leemans J (1992) Plant Cell 4:1495–1505

    Google Scholar 

  • Finer JJ, Vain P, Jones MW, McMullen MD (1992) Plant Cell Rep 11:323–328

    Google Scholar 

  • Franks T, Birch RG (1991) Aust J Plant Physiol 18:471–480

    Google Scholar 

  • Fromm ME, Taylor LP, Walbot V (1986) Nature 319:791–793

    Google Scholar 

  • Gambley RL, Ford R, Smith GR (1993) Plant Cell Rep 12:343–346

    Google Scholar 

  • Gordon-Kamm WJ, Spencer TM, Mangano ML, Adams TR Daines RJ, Start WG, O'Brien JV, Chambers SA, Adams WR Jr, Willetts NG, Rice TB, Mackey CJ, Krueger RW, Rausch AP, Lemaux PG (1990) Plant Cell 2:603–618

    Google Scholar 

  • Gould J, Devey M, Hasegawa O, Ulian EC, Peterson G, Smith RH (1991) Plant Physiol 95:426–434

    Google Scholar 

  • Hagio T, Blowers AD, Earle ED (1991) Plant Cell Rep 10:260–264

    Google Scholar 

  • Heinz DJ, Krishnamurthi M, Nickell LG, Maretzki A (1977) In:Reinert J, Bajaj VPS (eds) Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture. Springer-Verlag, Berlin, pp 3–17

    Google Scholar 

  • Jefferson RA (1987) Plant Mol Biol Rep 5:387–405

    CAS  Google Scholar 

  • Jefferson RA, Kavanagh TA, Bevan MW (1987) EMBO J 6:3901–3907

    CAS  PubMed  Google Scholar 

  • Klee H, Horsch R, Rogers S (1987) Ann Rev Plant Physiol 38:467–486

    Google Scholar 

  • Klein TM, Harper EC, Svab Z, Sanford JC, Fromm ME, Maliga, P (1988) Proc Natl Acad Sci USA 85:8502–8505

    Google Scholar 

  • Koziel MG, Beland GL, Bowman C, Carozzi NB, Crenshaw R, Crossland L, Dawson J, Desai N, Hill M, Kadwell S, Launis K, Lewis K, Maddox D, McPherson K, Meghji MR, Merlin E, Rhodes R, Warren GW, Wright M, Evola SV (1993) Bio/Technol 11:194–200

    Google Scholar 

  • Last DI, Brettell RIS, Chamberlain DA, Chaudhury AM, Larkin PJ, Marsh EL, Peacock WJ, Dennis ES (1991) Theor Appl Genet 81:581–588

    CAS  Google Scholar 

  • Luehrsen KR, Walbot V (1991) Mol Gen Genet 225:81–93

    Google Scholar 

  • Maas C, Werr W (1989) Plant Cell Rep 8:148–151

    Google Scholar 

  • McElroy D, Blowers AD, Jenes B, Wu R (1991) Mol Gen Genet 231:150–160

    Google Scholar 

  • McElroy D, Zhang W, Cao J, Wu R (1990) Plant Cell 2:163–171

    Article  CAS  PubMed  Google Scholar 

  • Meijer EGM, Schilperoort RA, Rueb S, van Os-Ruygrok PE, Hensgens LAM (1991) Plant Mol Biol 16:807–820

    Google Scholar 

  • Mooney PA, Goodwin PB, Dennis ES, Llewellyn DJ (1991) Plant Cell Tiss Org Culture 25:209–218

    Google Scholar 

  • Norris SR, Meyer SE, Callis J (1993) Plant Mol Biol 21:895–906

    Google Scholar 

  • Raineri DM, Bottini P, Gordon MP, Nester EW (1990) Bio/Technol 8:33–38

    Google Scholar 

  • Rathus C, Birch RG (1992) Plant Sci 82:81–89

    Google Scholar 

  • SAS Institute (1985) SAS user's guide: statistics. SAS Institute, Cary, N.C.

    Google Scholar 

  • Takeuchi Y, Dotson M, Keen NT (1992) Plant Mol Biol 18:835–9

    Google Scholar 

  • Toki S, Takamatsu S, Nojiri C, Ooba S, Anzai H, Iwata M, Christensen AH, Quail PH, Uchimiya H (1992) Plant Physiol 100:1503–1507

    Google Scholar 

  • Vasil V, Clancy M, Ferl RJ, Vasil IK, Hannah LC (1989) 91:1575–1579

  • Vasil V, Castillo AM, Fromm ME, Vasil IK (1992) Bio/Technol 10:667–674

    Google Scholar 

  • Zhang W, McElroy D, Wu R (1991) Plant Cell 3:1155–1165

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Agricultural Experiment Station, Texas A & M University, 2415 East Highway 83, 78596, Weslaco, TX, USA

    Maria Gallo-Meagher & James E. Irvine

Authors
  1. Maria Gallo-Meagher
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. James E. Irvine
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by G. C. Phillips

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gallo-Meagher, M., Irvine, J.E. Effects of tissue type and promoter strength on transient GUS expression in sugarcane following particle bombardment. Plant Cell Reports 12, 666–670 (1993). https://doi.org/10.1007/BF00233416

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00233416

Keywords

Search

Navigation