Abstract
Seeds of trifoliate orange (Poncirus trifoliata (L.) Raf.) are sensitive to desiccation, and could not withstand reduction in moisture level below 20%, whereas the excised embryonic axes could be easily desiccated to moisture levels as low as 14% without much loss in viability. Axes could be successfully cryopreserved in liquid nitrogen (−196°C) for eight months. The viable embryonic axes exhibited good growth on modified Murashige and Skoog medium supplemented wiith 1-Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP). Growth of cryopreserved axes was promoted in the presence of charcoal in the medium allowing for plant recovery.
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Abbreviations
- NAA:
-
Napthaleneacetic acid
- BAP:
-
6-Benzylaminopurine
- MS:
-
Murashige and Skoog (1962)
- LN:
-
Liquid nitrogen
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Communicated by G. C. Phillips
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Radhamani, J., Chandel, K.P.S. Cryopreservation of embryonic axes of trifoliate orange (Poncirus trifoliata [L.] RAF.). Plant Cell Reports 11, 372–374 (1992). https://doi.org/10.1007/BF00233369
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DOI: https://doi.org/10.1007/BF00233369