Summary
Hairy root cultures of Valeriana officinalis var. sambucifolia were established by infection of sterile plantlets with Agrobacterium rhizogenes strain R1601 The transformed roots were grown in 10 different, hormone-free liquid media and the isovaltrate, valtrate, didrovaltrate, isovaleroxyhydroxydidrovaltrate content was quantified by HPLC. Valepotriates were entirely retained inside the root tissues. The highest overall valepotriate content (10.3 % dry wt), 4 times the amount found in the roots of 9-month-old nontransformed plants, was observed in half strength Gamborg B5 medium supplemented with 2 % sucrose. The hairy roots cultured in Murashige and Skoog liquid medium supplemented with 2 % sucrose for 50 days produced over 44 mg/g dry wt valepotriates.
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Abbreviations
- MS:
-
Murashige and Skoog medium (Murashige and Skoog 1962)
- B5:
-
Gamborg B5 medium (Gamborg 1970)
- WP:
-
McCown's woody plant medium (Lloyd and McCown 1980)
- 1/2 MS-2:
-
half strength MS+2 % sucrose
- 1/4 B5-2:
-
quarter strength B5+2% sucrose
- MS-7:
-
full strength MS+7% sucrose
- YMB:
-
Yeast mannitol broth (Hooykaas et al. 1977)
- IVAL:
-
isovaltrate
- VAL:
-
valtrate
- IVHD:
-
isovaleroxyhydroxydidrovaltrate
- DI:
-
didrovaltrate (Fig. 1)
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Communicated by P. Matile
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Gränicher, F., Christen, P. & Kapetanidis, I. High-yield production of valepotriates by hairy root cultures of Valeriana officnalis L. var. sambucifolia Mikan. Plant Cell Reports 11, 339–342 (1992). https://doi.org/10.1007/BF00233361
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DOI: https://doi.org/10.1007/BF00233361