Abstract
A simple and reproducible protocol for regeneration of sugarbeet plants from hypocotyl expiants derived from 21 day-old-seedlings has been developed. Expiants were cultured on MS medium containing 0.3 mg/l N6-Benzylaminopurine, 0.1 mg/l Naphthalene Acetic Acid, 50 mg/l adenine and 0.5% (w/v) fructose, 0.5% (w/v) sucrose and 0.5% (w/v) glucose to induce the formation of organogenic calli (2.3% to 46.5% organogenic efficiency, depending on populations). Shoot formation was induced in callus cultures of more than 1600 genotypes. Physiological age affected culture response and different genotypes had different temperature optima for organogenesis. Following transfer of regenerated plants to the greenhouse, DNA determinations were made to study the stability of ploidy. Differences in ploidy were observed in plants derived from both shortterm and long-term callus cultures; diploid true-to-type regenerants were 96% and 83%, respectively, from shortterm and long-term callus cultures.
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Abbreviations
- MS:
-
Murashige and Skoog medium
- BAP:
-
N6-benzylaminopurine
- IBA:
-
Indolebutyric acid
- NAA:
-
Naphthalene acetic acid
- TIBA:
-
2,3,5 triiodobenzoic Acid
- GM:
-
Germination Medium
- IM:
-
Induction Medium
- RG:
-
Regeneration Medium
- RM:
-
Rooting Medium
References
Atanassov A, Kikindonov T, Antonova G (1976). Proc. Int. Symp.: Experimental mutation in plants. Varna, Bulgarie 14–17 October, 1976 309–317.
Brown S, Bergounioux C, Tallet S, Marie D (1991a). Chapt 5.3. I Negrutiu and G Gharti-Chhetri (Eds) Birkhäuser, Basel, Zurich, 1991.
Brown S, Devaux P, Marie D, Bergounioux C, Petti P (1991b). Biofutur 105: 2–16.
Catlin D (1990) Plant Cell Rep 9: 285–288.
Catlin D, Ochoa O, Mac Cormick S, Quiros C (1988) Plant Cell Rep 7: 100–103.
D'Amato F (1990) Caryologia 43: 191–204.
De Greef W, Jacobs M (1979) Plant Sci. Lett. 17: 55–61.
De Laat A, Godhe W, Vogelzang M (1987) Plant Breeding 99: 303–307.
Detrez C, Sangwan RS, Sangwan-Norreel BS (1989) Theor. Appl. Genet. 77: 462–468.
Detrez C, Tétu T, Sangwan RS, Sangwan-Norreel BS (1988) J. Exp. Botany 39: 917–926.
Doley W, Saunders J (1989) Plant Cell Rep 8: 22–225.
Freytag AH, Anand SC, Rao-Arelli AP, Owens LD (1988) Plant Cell Rep 7: 30–34.
Galbraith D, Harkins K, Ayres N, Sharma D, Firoozabady E (1983) Science 220: 1049–1051.
Groose R, Bingham E (1984) Crop. Sci. 24: 655–658.
Hooker M, Nabors M (1977). Z. Pflanzenphysiol. 84: 237–246.
Krens FA, Jamar D (1989) J. Plant Physiol. 134: 651–655.
Konwar B, Coutts R (1990) In Nijkamp H.J.J, Van Der Plas L.H.W., Van Aartrijk J. (Eds.). Kluwer Academic Publishers, Dordrecht, pp114–118.
Meins F (1983) Annu. Rev. Plant Physiol. 34: 327–346.
Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497.
Nagarajan P, Walton P (1987) Plant Cell Rep 6: 109–113.
Ritchie G, Short K, Davey M (1989) J. Exp. Bot. 211: 277–283.
Saunders J, Doley W (1986) J. Plant Physiol. 124: 473–479.
Saunders J, Mahomey M (1982) Euphytica 31: 801–804.
Saunders J, Shin K (1986) Crop Science 26: 1240–1245.
Tétu T (1989) Ph D thesis Université Paris VI, 137pp.
Tétu T, Sangwan RS, Sangwan-Norreel BS (1987) J. Exp. Bot. 38: 506–517.
Van Geyt J, Jacobs M (1985) Plant Cell Rep 4: 66–69.
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communicated by M. R. Davey
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Jacq, B., Tétu, T., Sangwan, R.S. et al. Plant regeneration from sugarbeet (Beta vulgaris L.) hypocotyls cultured in vitro and flow cytometric nuclear DNA analysis of regenerants. Plant Cell Reports 11, 329–333 (1992). https://doi.org/10.1007/BF00233359
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DOI: https://doi.org/10.1007/BF00233359