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Phenylacetic acid induced organogenesis in cultured leaf segments of Dianthus chinensis

Plant Cell Reports volume 15, pages 869–872 (1996)Cite this article

Summary

Shoot regeneration was achieved from leaf derived callus of Dianthus chinensis using Phenylacetic acid (PAA). Callus from basal leaf segments, raised on Murashige and Skoog's (MS) medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) or 1-Naphthaleneacetic acid (NAA) in combination with 6-benzylamino purine (BAP), was subcultured on medium supplemented with BAP in combination with 2,4-D, NAA or PAA. Shoots were induced only when leaf derived callus was subcultured on medium containing BAP (2.0, 5.0 mg/l) in combination with PAA (0.5, 1.0 mg/l). No shoot regeneration was observed when 2,4-D, NAA or BAP were used in the medium either singly or in different combinations. These results demonstrate that PAA in combination with BAP was essential to trigger shoot regeneration from cultured leaf callus of D. chinensis.

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Abbreviations

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

DPX:

dibutylphthalate xylol

MS:

Murashige and Skoog (1962) basal medium

NAA:

1-Naphthaleneacetic acid

PAA:

Phenylacetic acid

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Affiliations

  1. Department of Botany, University of Rajasthan, 302 004, Jaipur, India

    Vidya Jethwani & S. L. Kothari

Authors
  1. Vidya Jethwani
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  2. S. L. Kothari
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Communicated by G. C. Phillips

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Jethwani, V., Kothari, S.L. Phenylacetic acid induced organogenesis in cultured leaf segments of Dianthus chinensis . Plant Cell Reports 15, 869–872 (1996). https://doi.org/10.1007/BF00233159

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  • DOI: https://doi.org/10.1007/BF00233159

Keywords

  • Purine
  • Shoot Regeneration
  • Basal Leaf
  • Leaf Segment
  • Phenylacetic Acid