Abstract
Immature endosperm of Acacia nilotica formed a nodular callus on MS medium supplemented with 2,4-D, BAP and CH. In the third passage on this medium, in the dark, the callus differentiated somatic embryos. The embryos germinated on MS only after 15 d pre-treatment on modified MS medium in which major salts were replaced by those of major salts of B5 medium and supplemented with glutamine, CH and CW. Triploid nature of the somatic embryos was confirmed by Feulgen cytophotometry.
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- ABA:
-
abscisic acid
- AC:
-
activated charcoal
- BAP:
-
6-benzylaminopurine
- B5 :
-
Gamborg et al. (1968) medium
- CH:
-
casein hydrolysate
- CW:
-
coconut water
- d:
-
days
- MS:
-
Murashige and Skoog (1962) medium
- PEG 4000:
-
polyethylene glycol
- MW:
-
3500–4000
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
References
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Communicated by G. C. Phillips
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Garg, L., Bhandari, N.N., Rani, V. et al. Somatic embryogenesis and regeneration of triploid plants in endosperm cultures of Acacia nilotica . Plant Cell Reports 15, 855–858 (1996). https://doi.org/10.1007/BF00233156
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DOI: https://doi.org/10.1007/BF00233156