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Effect of promoter-leader sequences on transient expression of reporter gene chimeras biolistically transferred into sugarbeet (Beta vulgaris) suspension cells

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Summary

Chimeric constructs consisting of the gus coding region fused downstream of promoterun-translated leader sequences from the tobacco osmotin and PR-S genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (CaMV) 35S promoter were biolistically transferred into sugarbeet suspension cells. Each construct was expressed in recipient cells at 6 h after bombardment with maximum levels observed between 12 and 48 h. Expression of the PR-S construct mimicked the time-course expression of the constitutively expressed 35S construct but reached levels almost 50% higher. The pin2-promoter construct was ultimately expressed at levels similar to that of PR-S. Expression of the osmotin promoter-leader construct was highest, reaching levels approximately 2.5-fold higher than those of the 35S construct.

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Abbreviations

FW:

fresh weight

PSI:

pounds per square inch

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Authors and Affiliations

  1. Plant Molecular Biology Laboratory, United States Department of Agriculture, Beltsville Agricultural Research Center, Building 006, 20705-2350, Beltsville, Maryland, USA

    John C. Ingersoll, Thomas M. Heutte & Lowell D. Owens

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  1. John C. Ingersoll
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  2. Thomas M. Heutte
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  3. Lowell D. Owens
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Communicated by E. D. Earle

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Ingersoll, J.C., Heutte, T.M. & Owens, L.D. Effect of promoter-leader sequences on transient expression of reporter gene chimeras biolistically transferred into sugarbeet (Beta vulgaris) suspension cells. Plant Cell Reports 15, 836–840 (1996). https://doi.org/10.1007/BF00233152

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  • DOI: https://doi.org/10.1007/BF00233152

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