Skip to main content

Shoot regeneration in long-term callus cultures derived from mature flowering plants of Cyclamen persicum Mill.

Plant Cell Reports volume 15, pages 545–548 (1996)Cite this article

Summary

In long-term callus cultures of Cyclamen persicum Mill. two types of tissue could be distinguished. One type featured a brown suberised outer layer and was poorly organogenic. The other type was yellowish in appearance and gave rise to many shoot buds. Both types co-existed on the same callus, the former prevailing. Selection for organogenic tissue resulted in cultures yielding approximately three times more petioles than random subcultures. Callus-derived shoots could be rooted and established in the greenhouse. The method allowed for the production of thousands of plants but the regenerants often showed deviant phenotypes and genotypes.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Abbreviations

BA:

6-benzylaminopurine

BMP:

basal medium propagation

BMR:

basal medium rooting

DAPI:

4′,6-diamino-2-phenylindole

KIBA:

potassium salt of indole-3-butyric acid

kinetin:

6-furfurylaminopurine

MS:

Murashige and Skoog

NAA:

1-naphthaleneacetic acid

References

  • Dohm A, Schwenkel HG, Grunewaldt J (1991) Gartenbauwissenschaft 56:58–65

    Google Scholar 

  • Dunn B and Wobbe CR (1993) In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology, vol 2. John Wiley & Sons, New York, pp 13.13.1–13.13.9

    Google Scholar 

  • Fitch MMM, Moore PH (1990) Plant Cell, Tissue and Organ Culture 20:157–163

    Google Scholar 

  • Geier T (1977) Acta Hort 78:167–174

    Google Scholar 

  • Geier T, Kohlenbach HW, Reuter G (1990) In: Handbook of plant cell culture, 5. (eds) Ammirato PV, Evans DR, Sharp WR, Bajaj YPS. pp 352–374

  • Heyser JW and Nabors MW (1982) Crop Science 22:1070–1074

    Google Scholar 

  • Loewenberg JR (1969) Can J Bot 47:2065–2067

    Google Scholar 

  • Lu C, Vasil IK, Ozias-Akins P (1982) Theor Appl Genet 62:109–112

    Google Scholar 

  • Kiviharju E, Tuominen U, Törmälä T (1992) Plant Cell, Tissue and Organ Culture 28:187–194

    Google Scholar 

  • Murasaki K and Tsurushima H (1988) Acta Hort 226:721–724

    Google Scholar 

  • Murashige T and Skoog F (1962) Physiol Plant 15:473–479

    CAS  Google Scholar 

  • Nitsch JP (1969) Phytomorphology 19:389–404

    Google Scholar 

  • Redway FA, Vasil V, Lu D, Vasil IK (1990) Theor Appl Genet 79:609–617

    Google Scholar 

  • Schwenkel HG, Grunewaldt J (1991) Gartenbauwissenschaft 56:16–20

    Google Scholar 

  • Swedlund B, Locy RD (1988) Plant Cell Rep 7:144–147

    Google Scholar 

  • Wicart G, Mouras A, Lutz A (1984) Protoplasma 119:159–16

    Google Scholar 

Download references

Author information

Author notes

  1. Willy Dillen

    Present address: Laboratorium voor Genetica, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000, Gent, Belgium

Affiliations

  1. S&G Seeds B.V., Westeinde 62/P.O.Box 26, NL-1600, AA Enkhuizen, The Netherlands

    Willy Dillen, Ingrid Dijkstra & Johan Oud

Authors
  1. Willy Dillen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ingrid Dijkstra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Johan Oud
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by K. Glimelius

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Dillen, W., Dijkstra, I. & Oud, J. Shoot regeneration in long-term callus cultures derived from mature flowering plants of Cyclamen persicum Mill.. Plant Cell Reports 15, 545–548 (1996). https://doi.org/10.1007/BF00232991

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232991

Key words

  • Primulaceae
  • tissue culture
  • regeneration
  • somaclonal variation
  • organogenesis