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Somatic embryogenesis and plant regeneration from leaf derived callus of winged bean [Psophocarpus tetragonolobus (L.) DC.]

Plant Cell Reports volume 15, pages 531–535 (1996)Cite this article

Abstract

Somatic embryos were obtained from callus cultures derived from leaf explants of the winged bean, Psophocarpus tetragonolobus (L.) DC. Initiation and development of the somatic embryos occurred with a two-step culture method. Callus cultures initiated on MS medium with NAA and BAP, upon transfer to a new medium with IAA and BAP, produced somatic embryos. Maximum embryogenesis of 60% was obtained on induction medium with 0.5 mg/l NAA plus 1.0 mg/l BAP followed by transfer to a secondary medium with 0.1 mg/l IAA and 2.0 mg/l BAP. Optimal embryo germination and plantlet development was achieved on MS medium with 0.2 mg/l BAP plus 0.1 mg/l IBA. The regenerated plants were successfully transferred to glasshouse conditions.

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Abbreviations

MS:

Murashige and Skoog (1962) medium

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

1-naphthaleneacetic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

BAP:

6-benzylaminopurine

KN:

Kinetin

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Affiliations

  1. Department of Agricultural and Food Engineering, Indian Institute of Technology, 721302, Kharagpur, India

    Rina Ahmed, S. Dutta Gupta & Deepesh N. De

Authors
  1. Rina Ahmed
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  2. S. Dutta Gupta
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  3. Deepesh N. De
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Communicated by G. C. Phillips

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Ahmed, R., Dutta Gupta, S. & De, D.N. Somatic embryogenesis and plant regeneration from leaf derived callus of winged bean [Psophocarpus tetragonolobus (L.) DC.]. Plant Cell Reports 15, 531–535 (1996). https://doi.org/10.1007/BF00232988

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  • DOI: https://doi.org/10.1007/BF00232988

Keywords

  • Somatic Embryo
  • Somatic Embryogenesis
  • Plant Regeneration
  • Culture Method
  • Induction Medium