Skip to main content
Log in

Acetylsalicylic acid enhances and synchronizes thidiazuron-induced somatic embryogenesis in geranium (Pelargonium x hortorum Bailey) tissue cultures

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Summary

Thidiazuron (TDZ) effectively induced somatic embryogenesis in cultured hypocotyl explants of geranium (Pelargonium x hortorum Bailey) during only a 3-day period of induction. The presence of acetylsalicylic acid (ASA) during this period caused a two-fold increase in the number of somatic embryos and enhanced synchronization of embryo development compared to the TDZ treatment alone. Salicylic acid was ineffective in modulating similar embryogenic responses as ASA. The ASA-induced enhancement and synchronization of somatic embryogenesis could possibly be used as an experimental system to study the interplay of growth regulators in somatic embryogenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ASA:

acetylsalicylic acid

SA:

salicylic acid

References

  • Christianson ML, Warnick DA (1988) Organogenesis in vitro as a Developmental Process. HortScience 23:515–519.

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50:151–158.

    Google Scholar 

  • Gill R, Gerrath JM, Saxena PK (1993) High frequency direct somatic embryogenesis in thin layer cultures of hybrid seed geranium (Pelargonium x hortorum). Can. J. Bot. 71:408–413.

    Google Scholar 

  • Komamine A, Kawara R, Matsumoto M, Sunabori S, Toya T, Fujimura T (1992) Mechanisms of somatic embryogenesis in cell cultures: physiology, biochemistry and molecular biology. In Vitro Cell Dev. Biol. 28:11–14.

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497.

    Google Scholar 

  • Raskin I (1992) Role of salicylic acid in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43:439–463.

    Google Scholar 

  • Visser C, Qureshi JA, Gill R, Saxena PK (1992) Morphoregulatory role of Thidiazuron: substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl cultures. Plant Physiol. 99:1704–1707.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by F. Constabel

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hutchinson, M.J., Saxena, P.K. Acetylsalicylic acid enhances and synchronizes thidiazuron-induced somatic embryogenesis in geranium (Pelargonium x hortorum Bailey) tissue cultures. Plant Cell Reports 15, 512–515 (1996). https://doi.org/10.1007/BF00232984

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232984

Keywords

Navigation