Summary
A stabilized two-year old suspension of a Datura innoxia cell line, producing small amounts of tropane alkaloids (scopolamine and hyoscyamine) was used in this study. Calcium alginate immobilization has been shown to be able to increase secondary metabolite (i. e. alkaloid) production. The effects of calcium and ungellified alginate were both beneficial for tropane alkaloid synthesis; a 10mM calcium chloride supply gave the best results, with a 10-fold yield increase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- S:
-
standard cell culture medium
- HPLC:
-
high performance liquid chromatography
- FW:
-
fresh weight
- FDA:
-
fluorescein diacetate
- FW:
-
fresh weight
References
Ballica R, Ryu DDY and Kado C (1992) Biotechnol. Bioeng., 41, 1075–1081.
Brodelius P (1983) in: B Mattiasson Ed., CRC Press, Immobilized cells and organelles, Vol. 1, 27–55.
Engvild KC (1974) Physiol. Plant, 32, 390–393.
Flores HE and Galston AW (1982) Plant Physiol., 69, 701–706.
Gontier E (1993) Thèse de l'Université de Picardie Jules Verne, France, pp 200.
Gontier E, Fliniaux MA, Barbotin JN and Sangwan BS (1993) Planta Med., 59, 432–435.
Hashimoto T and Yamada Y (1987) Agric. Biol. Chem., 51, 2769–2774.
Hashimoto T, Yukimune Y and Yamada Y (1989) Planta, 178, 123–130.
Herouart D, Sangwan RS and Sangwan BS (1991) Plant Cell Tissue and Organ Cult., 24, 97–104.
Hulst AC and Tramper J (1989) Enz. Microb. Technol., 11, 546–558.
Jackson PJ, Torres AP, Delhaize E, Pack E and Bolender SL (1990) J. Environ. Qual., 19, 644–648.
Karel S, Libicki SB and Robertson CR (1985) Chem. Eng. Sc., 40, 1321–1354.
Kibler R and Neumann KH (1979) Planta Med., 35, 354–359.
Kitamura Y, Miura H and Sugii M (1985) Chem. Pharm. Bull., 33, 5445–5448.
Knorr D (1986) In: Knorr D Ed., Food Biotechnology, Marcel Dekker Inc, USA, 287–319.
Marguery P (1992) Thèse de l'Université de Picardie Jules Verne, France, pp 200.
Murashige T and Skoog F (1962) Physiol. Plant., 15, 473–497.
Nakajima H, Sonomoto K, Usui N, Sato F, Yamada Y, Tanaka A and Fukui S (1985) J. Biotechnol., 2, 107–117.
Nitsch JP and Nitsch C (1965) Ann. Physiol. vég., 7, 251–256.
Plank KH and Wagner KG (1986) Z. Naturforsch, 41c, 391–395.
Pras N (1992) J. Biotechnol., 26, 29–62.
Thomasset B, Gontier E, Sangwan BS and Barbotin JN (1990) in: JAM De Bont, J Visser, B Mattiasson and J Tramper Ed., Elsevier Science Publishers, Physiology of immobilized cells, 597–602.
Tseng A and Redmond RW (1970) J. Chrom., 170, 479–481.
Author information
Authors and Affiliations
Additional information
Communicated by W. Barz
Rights and permissions
About this article
Cite this article
Gontier, E., Sangwan, B.S. & Barbotin, J.N. Effects of calcium, alginate, and calcium-alginate immobilization on growth and tropane alkaloid levels of a stable suspension cell line of Datura innoxia Mill. Plant Cell Reports 13, 533–536 (1994). https://doi.org/10.1007/BF00232951
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232951