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Production of L-DOPA from cell suspension culture of Mucuna pruriens f. pruriens

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Summary

Production of L-DOPA was studied in cell suspension culture of Mucuna pruriens f. pruriens. Suspension culture was established in MSI medium composed of half concentration of Murashige and Skoog's salts and 2% sucrose. A two-stage cell suspension culture was developed for enhanced accumulation of L-DOPA. In the first stage, the culture system was composed of MSI medium without CaCl, which was suitable for cell growth and in the second stage MSI medium containing 42.5 mg.l−1 KH2PO4 and 4% sucrose favoured L-DOPA production. A discernible higher production of L-DOPA was obtained in this two-stage cell suspension culture in comparison to single stage culture.

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References

  • Berntsson PB, Gaarer JO, Lam BR (1973) Ger Often 2: 264–390.

    Google Scholar 

  • Brain KR (1976) Plant Sci Lett 7: 157–161.

    Google Scholar 

  • British Pharmacopoeia (1973) London.

  • Chattopadhyay S, Das A (1990), FEMS Micro Lett 72: 195–200.

    Google Scholar 

  • Chattopadhyay S, Datta SK (1985) Acta Horti 188s: 51–55.

    Google Scholar 

  • Daxenbichler ME, Van Etten CH, Halliman EA, Earle FR (1971) J Med Chem 14: 463–465.

    Google Scholar 

  • Griffith T, Conn E (1973) Phytochem 12: 1651–1656.

    Google Scholar 

  • Guggenheim M (1913) Z Physiol Chem 88: 276.

    Google Scholar 

  • Huizing HJ, Wijnsma R, Batterman S, Malingre Th.M, Wichers HJ (1985) Plant Cell Tiss Org Cul 4: 61–74.

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15: 473.

    CAS  Google Scholar 

  • Obata-Sasamoto H, Komamine A (1983) Planta Medica 49: 120–123.

    Google Scholar 

  • Pramanik TK, Datta SK (1986) Plant Cell Reports 3: 219–222.

    Google Scholar 

  • Remmen SFA, Ellis BE (1980) Phytochem 19: 1421–1423.

    Google Scholar 

  • Sih SJ, Foss P, John R, Michal L (1969) J Am Chem Soc 91: 6204.

    Google Scholar 

  • Wichers, HJ, Wijnsma R, Visser JF, Malingre TM, Huizing HJ (1985) Plant Cell Tiss Org Cult 4: 75–80.

    Google Scholar 

  • Yamada Y, Fujita Y (1983) In: Evans DA, Sharp WR, Ammirato PV (eds) Handbook of Plant Cell Culture, Vol.1, MacMillan, New York, pp 717–728.

    Google Scholar 

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Author notes

  1. Sharmila Chattopadhyay

    Present address: Department of Chemistry, Bose Institute, 93/1, APC Road, 700009, Calcutta, India

Authors and Affiliations

  1. Department of Botany, Visva Bharati University, Santiniketan, W.B., India

    Sharmila Chattopadhyay & S. K. Datta

  2. Indian Institute of Chemical Biology, 700032, Calcutta, India

    S. B. Mahato

Authors
  1. Sharmila Chattopadhyay
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  2. S. K. Datta
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  3. S. B. Mahato
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Communicated by A. R. Gould

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Chattopadhyay, S., Datta, S.K. & Mahato, S.B. Production of L-DOPA from cell suspension culture of Mucuna pruriens f. pruriens . Plant Cell Reports 13, 519–522 (1994). https://doi.org/10.1007/BF00232948

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  • DOI: https://doi.org/10.1007/BF00232948

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