Summary
Studies on uptake and assimilation of nitrate in plants are confounded by differences in cell function associated with anatomical features of roots as well as by problems inherent with growing plants without nitrate. To circumvent these problems, a Zea mays L. embryo cell line was grown in suspension culture using an amino-acid-based medium consisting of a Murashige and Skoog medium in which ammonium and nitrate were replaced by aspartic acid (100 mg/l), glycine (100 mg/l), arginine (150 mg/l), and glutamine (1 g/l). The growth, cellular characteristics, and physical appearance of the amino-acid-grown cells were similar to cells grown in the presence of nitrate. The amino-acid-grown cells exhibited the expected induction pattern and inhibitor sensitivity of nitrate uptake. This cell line should facilitate research on the induction of nitrate uptake and the regulation of nitrate assimilation into proteins.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Behrend J, Mateles RI (1975) Pknt Physiol 56: 584–589
Clark G (1981) Staining procedures. Williams and Wilkins, Baltimore, Maryland
Clarkson DT (1986) In: Lambers H, Neetson JJ, Stulen I (eds) Fundamental, ecological and agricultural aspects of nitrogen metabolism in higher plants. Nijhoff Publishers, Boston, MA, pp 3–27
CRC Handbook of Chromatography: Plant Pigments (1986) Part I: Carotenoids. CRC Publishers, Boca Raton, Florida
Deane-Drummond CE (1990) In: Abrol YP (ed) Nitrogen in higher plants. John Wiley and Sons, Inc, New York, pp 1–37
Dhugga KS, Waines JG, Leonard RT (1988) Plant Physiol 86: 759–763
Duncan DR, Widholm JM (1991) Maize Genet Coop Newsl 65: 114
Felker FC (1987) Amer J Bot 74: 1912–1920
Felker FC, Miernyk JA, Crawford CG (1989) Plant Cell Tissue Organ Cult 18: 153–166
Gamborg LO (1970) Plant Physiol 45: 372–375
Heimer YM, Filner P (1971) Biochim Biophys Acta 230: 362–372
Jackson WA, Flesher D, Hageman RH (1973) Plant Physiol 51: 120–127
Kriz AL, Schwartz D (1986) Plant Physiol 82: 1069–1075
Lamport DTA (1964) Exp Cell Res 33: 195–206
Mitchell DE, Gadus MV, Madore MA (1992) Plant Physiol 99: 959–965
Mohanty B, Fletcher JS (1978) Physiol Plant 42: 221–225
Murashige T, Skoog F (1962) Plant Physiol 15: 473–497
O'Brien TP, McCully ME (1981) The study of plant structure principles and selected methods. Termarcarphi Pty Ltd, Melbourne, Australia
Oaks A (1986) In: Lambers H, Neetson JJ, Stulen I (eds) Fundamental, ecological and agricultural aspects of nitrogen metabolism in higher plants. Nijhoff Publishers, Boston, MA, pp 133–151
Padgett PE, Leonard RT (1993) Plant Physiol 101: 141–146
Peterson GL (1977) Anal Biochem 83: 346–356
Reider ML, Fletcher JS (1986) Plant Cell, Tissue Organ Cult 7: 115–119
Rosen H (1957) Arch Biochem Biophys 67: 10–15
Rufty TW Jr, Thomas JF, Remmler JL, Campbell WH, Volk RJ (1986) Plant Physiol 82: 675–680
Scholl RL, Harper JE, Hageman RH (1974) Plant Physiol 53: 825–828
Shimamoto K, Ackerman M, Dierks-Ventling C (1983) Plant Physiol 73: 915–920
Skokut TA, Volk CP, Thomas J, Meeks JC, Shaffer PW (1978) Plant Physiol 62: 299–304
Zhang N, MacKown CT (1993) Plant Physiol 102: 851–857
Author information
Authors and Affiliations
Additional information
Communicated by J. M. Widholm
Rights and permissions
About this article
Cite this article
Padgett, P.E., Leonard, R.T. An amino-acid-grown maize cell line for use in investigating nitrate assimilation. Plant Cell Reports 13, 504–509 (1994). https://doi.org/10.1007/BF00232945
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232945