Summary
Genomic DNA (Aso1) encoding the ascorbate oxidase (ASO) of cucumber (Cucumis sativas) was cloned and sequenced. From a comparison of the sequences of Aso1 and its cDNA [Ohkawa et al. Proc. Natl. Acad. Sci. USA 86 (1989) 1239–1243], it was concluded that Aso1 consisted of four exons and three introns. In the 5′-flanking region, sequence motif resembling a TATA box and a CAAT box, were found 124 and 218 bp upstream, respectively, from the start codon. Sites of initiation of transcription, determined by primer extension, were the A and G residues located 90 and 93 bp, respectively, upstream from the initiation codon. ASO was abundant in cucumber fruits and stems. The 5′-flanking region, placed upstream of the structural gene of β-glucuronidase (GUS), expressed the GUS activity in cucumber plants in a transient expression assay after introduction of DNA with a particle gun. High-level GUS expression was found in stems of transgenic tobacco plants harboring Aso1 promoter-GUS chimeric gene.
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Communicated by R. L. Rodriguez
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Ohkawa, J., Ohya, T., Ito, T. et al. Structure of the genomic DNA encoding cucumber ascorbate oxidase and its expression in transgenic plants. Plant Cell Reports 13, 481–488 (1994). https://doi.org/10.1007/BF00232941
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DOI: https://doi.org/10.1007/BF00232941