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Intergeneric protoplast fusion between Brassica carinata and Camelina sativa

Plant Cell Reports volume 13, pages 657–660 (1994)Cite this article

Abstract

Camelina sativa is a wild crucifer that is reported to be resistant to Alternaria blight. Polyethylene glycol mediated fusion was attempted between protoplasts from etiolated hypocotyls of Brassica carinata and mesophyll protoplasts of Camelina sativa. The mean frequency of heterokaryons was 6.8%. Three hybrid shoots were regenerated, each from a single fusionderived callus. These shoots failed to produce roots capable of withstanding transplantation. Confirmation of hybridity was obtained from the morphology of in vitro produced leaves, somatic chromosome number in leaf tips, and restriction fragment length polymorphism for a nuclear rDNA probe. Analysis for organelle constitution using RFLPs indicated that the hybrid contained chrloroplasts derived from the wild species and mitochondria from the cultivated Brassica species.

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Abbreviations

2,4-D:

2,4-dichlorophenoxy-acetic acid

IAA:

Indole-3-acetic acid

NAA:

-Naphthaleneacetic acid

IBA:

Indole-3-butyric acid

GA3 :

gibberellic acid

BAP:

6-Benzylaminopurine

MS:

Murashige and Skoog (1962) basal medium

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Affiliations

  1. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India

    S. B. Narasimhulu, P. B. Kirti, Shyam Prakash & V. L. Chopra

  2. National Bureau of Plant Genetic Resources, New Delhi, India

    S. R. Bhatt

Authors
  1. S. B. Narasimhulu
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  2. P. B. Kirti
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  3. S. R. Bhatt
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  4. Shyam Prakash
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  5. V. L. Chopra
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Communicated by G. C. Phillips

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Narasimhulu, S.B., Kirti, P.B., Bhatt, S.R. et al. Intergeneric protoplast fusion between Brassica carinata and Camelina sativa . Plant Cell Reports 13, 657–660 (1994). https://doi.org/10.1007/BF00232940

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  • DOI: https://doi.org/10.1007/BF00232940

Keywords

  • Chromosome Number
  • Restriction Fragment Length Polymorphism
  • Fragment Length Polymorphism
  • Wild Species
  • Polyethylene Glycol