Summary
Cytogenetic examination of transgenic Arabidopsis thaliana (L.) Heynh. plants obtained by Agrobacterium-mediated gene transfer to cotyledon- and root-explants or by direct gene transfer into protoplasts revealed a high percentage of tetraploid or aneuploid transformants. Depending on the transformation procedure used, 13% (root explant transformation), 33% (cotyledon explant transformation), or 38% (direct gene transfer) of the transformants showed aberrant ploidy levels. A good correlation between the ploidy level of a plant and the size of its pollen grains was observed. This allows quick and simple testing of the ploidy level of transgenic Arabidopsis plants.
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Abbreviations
- AM:
-
Arabidopsis medium
- ANOVA:
-
analysis of variance
- DAPI:
-
4,6-Diamidino-2-phenylindole
- PEG:
-
polyethyleneglycol
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Altmann, T., Damm, B., Frommer, W.B. et al. Easy determination of ploidy level in Arabidopsis thaliana plants by means of pollen size measurement. Plant Cell Reports 13, 652–656 (1994). https://doi.org/10.1007/BF00232939
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DOI: https://doi.org/10.1007/BF00232939