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A simple procedure for the expression of genes in transgenic soybean callus tissue

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Abstract

Cotyledons from germinating seeds of the soybean cultivar Peking were inoculated with virulent Agrobacterium tumefaciens strain A281:pZA-7 which carries a wild type Ti plasmid pTiBo542 and a disarmed Ti plasmid (a binary vector)pZA-7 which contains the βglucuronidase (uidA) and neomycin phosphotransferase (nptII) genes. Tumors were produced on all inoculated explants and 82% of these tumor lines were cotransformed by the nptII gene from the binary vector pZA-7 as shown by PCR analysis (18 of 22 lines tested). Eleven of these 18 lines were also resistant to kanamycin. Eleven lines expressed β-glucuronidase activity (GUS), six of which were also kanamycin resistant. Since there is a high rate of coexpression of genes carried by the binary vector, this system provides a simple and rapid method for the expression of genes of interest in transformed soybean tissue which has been used successfully to test constructs designed for soybean transformation.

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Author notes

  1. G. Luo

    Present address: UT M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Box 45, 77030, Houston, TX, USA

  2. A. Hepburn

    Present address: Profile Diagnostic Sciences, Inc., 510 E. 73rd St., 10021, New York, NY

Authors and Affiliations

  1. Department of Agronomy, University of Illinois, PABL, 1102 W. Gregory, 61801, Urbana, IL, USA

    G. Luo, A. Hepburn & J. Widholm

Authors
  1. G. Luo
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  2. A. Hepburn
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  3. J. Widholm
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Communicated by R. Shoemaker

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Luo, G., Hepburn, A. & Widholm, J. A simple procedure for the expression of genes in transgenic soybean callus tissue. Plant Cell Reports 13, 632–636 (1994). https://doi.org/10.1007/BF00232935

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  • DOI: https://doi.org/10.1007/BF00232935

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