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A comparison of several published methods for barley anther culture

Plant Cell Reports volume 14, pages 763–767 (1995)Cite this article

Abstract

A number of methods have been published for barley (Hordeum vulgare L.) anther culture and have gained acceptance in different laboratories. The breeder's requirement is for a compromise method that gives good, repeatable results for a wide range of genotypes. Yet the routine production of spontaneously doubled haploid green regenerants remains difficult. Despite attempts to formulate a widely-applicable anther culture method, the 4 main published methods, compared here with one modified procedure, are quite distinct for a number of important characteristics. The methods interacted strongly with the 3 genotypes, and response ranged from zero to 28 green regenerants per 100 anthers plated. The current methods still require often substantial modification to suit local situations in order that the technology may be exploited by barley breeders.

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Abbreviations

BAP:

benzylaminopurine

DH:

doubled haploid

FV:

final volume

IAA:

indoleacetic acid

IBA:

indole-3-butyric acid

MS:

Murashige & Skoog

PABA:

para-aminobenzoic acid

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Affiliations

  1. NSW Agriculture, Agricultural Research Institute, Private Mail Bag, 2650, Wagga Wagga, NSW, Australia

    David J. Luckett & Ross A. Smithard

Authors
  1. David J. Luckett
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  2. Ross A. Smithard
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Communicated by E. D. Earle

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Luckett, D.J., Smithard, R.A. A comparison of several published methods for barley anther culture. Plant Cell Reports 14, 763–767 (1995). https://doi.org/10.1007/BF00232918

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  • DOI: https://doi.org/10.1007/BF00232918

Key words

  • androgenesis
  • doubled haploids
  • plant breeding
  • Hordeum vulgare L.